Lipopolysaccharides of anaerobic beer spoilage bacteria of the genus Pectinatus: Lipopolysaccharides of a Gram-positive genus

Ilkka Helander (Corresponding Author), Auli Haikara, Irina Sadovskaya, Evgeny Vinogradov, Mirja Salkinoja-Salonen

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Bacteria of the genus Pectinatus emerged during the seventies as contaminants and spoilage organisms in packaged beer. This genus comprises two species, Pectinatus cerevisiiphilus and Pectinatus frisingensis; both are strict anaerobes. On the basis of genomic properties the genus is placed among low GC Gram-positive bacteria (phylum Firmicutes, class Clostridia, order Clostridiales, family Acidaminococcaceae). Despite this assignment, Pectinatus bacteria possess an outer membrane and lipopolysaccharide (LPS) typical of Gram-negative bacteria. The present review compiles the structural and compositional studies performed on Pectinatus LPS. These lipopolysaccharides exhibit extensive heterogeneity, i.e. several macromolecularly and structurally distinct LPS molecules are produced by each strain. Whereas heterogeneity is a common property in lipopolysaccharides, Pectinatus LPS have been shown to contain exceptional carbohydrate structures, consisting of a fairly conserved core region that carries a large non-repetitive saccharide that probably replaces the O-specific chain. Such structures represent a novel architectural principle of the LPS molecule.
Original languageEnglish
Pages (from-to)543 - 552
Number of pages10
JournalFEMS Microbiology Reviews
Volume28
Issue number5
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Pectinatus
Lipopolysaccharides
Bacteria
Veillonellaceae
Clostridium
Gram-Positive Bacteria
Gram-Negative Bacteria
Carbohydrates

Keywords

  • Pectinatus
  • Lipopolysaccharide
  • Gram-positive
  • Beer spoilage

Cite this

Helander, Ilkka ; Haikara, Auli ; Sadovskaya, Irina ; Vinogradov, Evgeny ; Salkinoja-Salonen, Mirja. / Lipopolysaccharides of anaerobic beer spoilage bacteria of the genus Pectinatus : Lipopolysaccharides of a Gram-positive genus. In: FEMS Microbiology Reviews. 2004 ; Vol. 28, No. 5. pp. 543 - 552.
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abstract = "Bacteria of the genus Pectinatus emerged during the seventies as contaminants and spoilage organisms in packaged beer. This genus comprises two species, Pectinatus cerevisiiphilus and Pectinatus frisingensis; both are strict anaerobes. On the basis of genomic properties the genus is placed among low GC Gram-positive bacteria (phylum Firmicutes, class Clostridia, order Clostridiales, family Acidaminococcaceae). Despite this assignment, Pectinatus bacteria possess an outer membrane and lipopolysaccharide (LPS) typical of Gram-negative bacteria. The present review compiles the structural and compositional studies performed on Pectinatus LPS. These lipopolysaccharides exhibit extensive heterogeneity, i.e. several macromolecularly and structurally distinct LPS molecules are produced by each strain. Whereas heterogeneity is a common property in lipopolysaccharides, Pectinatus LPS have been shown to contain exceptional carbohydrate structures, consisting of a fairly conserved core region that carries a large non-repetitive saccharide that probably replaces the O-specific chain. Such structures represent a novel architectural principle of the LPS molecule.",
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Lipopolysaccharides of anaerobic beer spoilage bacteria of the genus Pectinatus : Lipopolysaccharides of a Gram-positive genus. / Helander, Ilkka (Corresponding Author); Haikara, Auli; Sadovskaya, Irina; Vinogradov, Evgeny; Salkinoja-Salonen, Mirja.

In: FEMS Microbiology Reviews, Vol. 28, No. 5, 2004, p. 543 - 552.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Lipopolysaccharides of anaerobic beer spoilage bacteria of the genus Pectinatus

T2 - Lipopolysaccharides of a Gram-positive genus

AU - Helander, Ilkka

AU - Haikara, Auli

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AU - Vinogradov, Evgeny

AU - Salkinoja-Salonen, Mirja

PY - 2004

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N2 - Bacteria of the genus Pectinatus emerged during the seventies as contaminants and spoilage organisms in packaged beer. This genus comprises two species, Pectinatus cerevisiiphilus and Pectinatus frisingensis; both are strict anaerobes. On the basis of genomic properties the genus is placed among low GC Gram-positive bacteria (phylum Firmicutes, class Clostridia, order Clostridiales, family Acidaminococcaceae). Despite this assignment, Pectinatus bacteria possess an outer membrane and lipopolysaccharide (LPS) typical of Gram-negative bacteria. The present review compiles the structural and compositional studies performed on Pectinatus LPS. These lipopolysaccharides exhibit extensive heterogeneity, i.e. several macromolecularly and structurally distinct LPS molecules are produced by each strain. Whereas heterogeneity is a common property in lipopolysaccharides, Pectinatus LPS have been shown to contain exceptional carbohydrate structures, consisting of a fairly conserved core region that carries a large non-repetitive saccharide that probably replaces the O-specific chain. Such structures represent a novel architectural principle of the LPS molecule.

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