Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

N. Szijártó (Corresponding Author), Matti Siika-aho, T. Sontag-Strohm, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15% (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/g dry solids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.
Original languageEnglish
Pages (from-to)1968-1974
Number of pages7
JournalBioresource Technology
Volume102
Issue number2
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Straw
Liquefaction
liquefaction
straw
Enzymes
wheat
enzyme
viscosity
Viscosity
Cellulose 1,4-beta-Cellobiosidase
substrate
Saccharification
Cellulase
Substrates
cleavage
hydrolysis
Hydrolysis
replacement
experiment
Experiments

Keywords

  • Biomass
  • liquefaction
  • cellulase
  • Trichoderma reesei

Cite this

Szijártó, N. ; Siika-aho, Matti ; Sontag-Strohm, T. ; Viikari, Liisa. / Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes. In: Bioresource Technology. 2010 ; Vol. 102, No. 2. pp. 1968-1974.
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abstract = "Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15{\%} (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/g dry solids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.",
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Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes. / Szijártó, N. (Corresponding Author); Siika-aho, Matti; Sontag-Strohm, T.; Viikari, Liisa.

In: Bioresource Technology, Vol. 102, No. 2, 2010, p. 1968-1974.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

AU - Szijártó, N.

AU - Siika-aho, Matti

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AU - Viikari, Liisa

PY - 2010

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N2 - Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15% (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/g dry solids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.

AB - Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15% (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/g dry solids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.

KW - Biomass

KW - liquefaction

KW - cellulase

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