Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

N. Szijártó (Corresponding Author), Matti Siika-aho, T. Sontag-Strohm, Liisa Viikari

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15% (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/g dry solids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.
    Original languageEnglish
    Pages (from-to)1968-1974
    Number of pages7
    JournalBioresource Technology
    Volume102
    Issue number2
    DOIs
    Publication statusPublished - 2010
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Biomass
    • liquefaction
    • cellulase
    • Trichoderma reesei

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