Abstract
In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Subtitle of host publication | Methods and Protocols |
| Editors | Kristian Spilling |
| Publisher | Humana Press |
| Pages | 215-222 |
| Number of pages | 8 |
| ISBN (Electronic) | 978-1-4939-9416-8 |
| ISBN (Print) | 978-1-4939-9415-1 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
| MoE publication type | A3 Part of a book or another research book |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 1980 |
| ISSN | 1064-3745 |
Fingerprint
Keywords
- lipid
- liquid chromatography
- mass spectrometry
Cite this
}
Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples. / Nygren, Heli; Seppänen-Laakso, Tuulikki; Rischer, Heiko.
Methods in Molecular Biology: Methods and Protocols. ed. / Kristian Spilling. Humana Press, 2020. p. 215-222 (Methods in Molecular Biology, Vol. 1980).Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review
TY - CHAP
T1 - Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples
AU - Nygren, Heli
AU - Seppänen-Laakso, Tuulikki
AU - Rischer, Heiko
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
AB - In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
KW - lipid
KW - liquid chromatography
KW - mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85076398655&partnerID=8YFLogxK
U2 - 10.1007/7651_2017_108
DO - 10.1007/7651_2017_108
M3 - Chapter or book article
SN - 978-1-4939-9415-1
T3 - Methods in Molecular Biology
SP - 215
EP - 222
BT - Methods in Molecular Biology
A2 - Spilling, Kristian
PB - Humana Press
ER -