Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationMethods and Protocols
EditorsKristian Spilling
PublisherHumana Press
Pages215-222
Number of pages8
ISBN (Electronic)978-1-4939-9416-8
ISBN (Print)978-1-4939-9415-1
DOIs
Publication statusPublished - 1 Jan 2020
MoE publication typeA3 Part of a book or another research book

Publication series

SeriesMethods in Molecular Biology
Volume1980
ISSN1064-3745

Keywords

  • lipid
  • liquid chromatography
  • mass spectrometry

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  • Cite this

    Nygren, H., Seppänen-Laakso, T., & Rischer, H. (2020). Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples. In K. Spilling (Ed.), Methods in Molecular Biology: Methods and Protocols (pp. 215-222). Humana Press. Methods in Molecular Biology, Vol.. 1980 https://doi.org/10.1007/7651_2017_108