Abstract
In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Subtitle of host publication | Methods and Protocols |
| Editors | Kristian Spilling |
| Publisher | Humana Press |
| Pages | 215-222 |
| Number of pages | 8 |
| ISBN (Electronic) | 978-1-4939-9416-8 |
| ISBN (Print) | 978-1-4939-9415-1 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
| MoE publication type | A3 Part of a book or another research book |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 1980 |
| ISSN | 1064-3745 |
Keywords
- lipid
- liquid chromatography
- mass spectrometry
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Cite this
Nygren, H., Seppänen-Laakso, T., & Rischer, H. (2020). Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples. In K. Spilling (Ed.), Methods in Molecular Biology: Methods and Protocols (pp. 215-222). Humana Press. Methods in Molecular Biology, Vol.. 1980 https://doi.org/10.1007/7651_2017_108