Liquid chromatography-mass spectrometry (LC-MS)-based analysis of molecular lipids in algae samples

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    1 Citation (Scopus)

    Abstract

    In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    Subtitle of host publicationMethods and Protocols
    EditorsKristian Spilling
    PublisherHumana Press
    Pages215-222
    Number of pages8
    ISBN (Electronic)978-1-4939-9416-8
    ISBN (Print)978-1-4939-9415-1
    DOIs
    Publication statusPublished - 1 Jan 2020
    MoE publication typeA3 Part of a book or another research book

    Publication series

    SeriesMethods in Molecular Biology
    Volume1980
    ISSN1064-3745

    Keywords

    • lipid
    • liquid chromatography
    • mass spectrometry

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