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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples Using Data Dependent Acquisition (DDA)

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For the characterization of lipids, data dependent acquisition (DDA) analyses are carried out utilizing a high-resolution quadrupole-time-of-flight (Q-ToF) instrument. Throughput of the method is over 100 samples/day. The repeatability is good, and the relative standard deviation of spiked samples is <15%.

Original languageEnglish
Title of host publication Biofuels from Algae
EditorsKristian Spilling
PublisherHumana Press
Pages71-78
ISBN (Electronic)978-1-0716-5031-8
ISBN (Print)978-1-0716-5030-1
DOIs
Publication statusPublished - 2026
MoE publication typeA3 Part of a book or another research book

Publication series

SeriesMethods in Molecular Biology
Volume2996
ISSN1064-3745

Keywords

  • Data dependent acquisition
  • Lipid
  • Liquid chromatography
  • Mass spectrometry

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