Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

Heli Nygren; Tuulikki Seppänen-Laakso; Sandra Castillo; Tuulia Hyötyläinen; Matej Oresic

doi:10.1007/978-1-61737-985-7_15

Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Oresic

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

91 Citations (Scopus)

Abstract

In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.

Original language	English
Title of host publication	Metabolic Profiling
Subtitle of host publication	Methods and Protocols
Editors	Thomas O. Metz
Publisher	Springer
Chapter	15
Pages	247-257
ISBN (Electronic)	978-1-61737-985-7
ISBN (Print)	978-1-61737-984-0
DOIs	https://doi.org/10.1007/978-1-61737-985-7_15
Publication status	Published - 2011
MoE publication type	A3 Part of a book or another research book

Keywords

Lipidomics
liquid chromatography
mass spectrometry

Access to Document

10.1007/978-1-61737-985-7_15

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Cite this

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abstract = "In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.",

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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues. / Nygren, Heli ; Seppänen-Laakso, Tuulikki ; Castillo, Sandra; Hyötyläinen, Tuulia; Oresic, Matej.

Metabolic Profiling: Methods and Protocols. ed. / Thomas O. Metz. Springer, 2011. p. 247-257.

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

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AU - Hyötyläinen, Tuulia

AU - Oresic, Matej

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