@inbook{07c00591e76648c88549cb640fc955e9,
title = "Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues",
abstract = "In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.",
keywords = "Lipidomics, liquid chromatography, mass spectrometry",
author = "Heli Nygren and Tuulikki Sepp{\"a}nen-Laakso and Sandra Castillo and Tuulia Hy{\"o}tyl{\"a}inen and Matej Ore{\v s}i{\v c}",
year = "2011",
doi = "10.1007/978-1-61737-985-7_15",
language = "English",
isbn = "978-1-61737-984-0",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "247--257",
editor = "Metz, {Thomas O.}",
booktitle = "Metabolic Profiling",
address = "Germany",
}