In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
|Title of host publication||Metabolic Profiling|
|Subtitle of host publication||Methods and Protocols|
|Editors||Thomas O. Metz|
|Publication status||Published - 2011|
|MoE publication type||A3 Part of a book or another research book|
- liquid chromatography
- mass spectrometry