Abstract
| Original language | English |
|---|---|
| Title of host publication | Metabolic Profiling |
| Subtitle of host publication | Methods and Protocols |
| Editors | Thomas O. Metz |
| Publisher | Springer |
| Chapter | 15 |
| Pages | 247-257 |
| ISBN (Electronic) | 978-1-61737-985-7 |
| ISBN (Print) | 978-1-61737-984-0 |
| DOIs | |
| Publication status | Published - 2011 |
| MoE publication type | A3 Part of a book or another research book |
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Keywords
- Lipidomics
- liquid chromatography
- mass spectrometry
Cite this
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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues. / Nygren, Heli; Seppänen-Laakso, Tuulikki; Castillo, Sandra; Hyötyläinen, Tuulia; Oresic, Matej.
Metabolic Profiling: Methods and Protocols. ed. / Thomas O. Metz. Springer, 2011. p. 247-257.Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review
TY - CHAP
T1 - Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues
AU - Nygren, Heli
AU - Seppänen-Laakso, Tuulikki
AU - Castillo, Sandra
AU - Hyötyläinen, Tuulia
AU - Oresic, Matej
PY - 2011
Y1 - 2011
N2 - In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
AB - In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
KW - Lipidomics
KW - liquid chromatography
KW - mass spectrometry
U2 - 10.1007/978-1-61737-985-7_15
DO - 10.1007/978-1-61737-985-7_15
M3 - Chapter or book article
SN - 978-1-61737-984-0
SP - 247
EP - 257
BT - Metabolic Profiling
A2 - Metz, Thomas O.
PB - Springer
ER -