Abstract
In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
| Original language | English |
|---|---|
| Title of host publication | Metabolic Profiling |
| Subtitle of host publication | Methods and Protocols |
| Editors | Thomas O. Metz |
| Publisher | Springer |
| Chapter | 15 |
| Pages | 247-257 |
| ISBN (Electronic) | 978-1-61737-985-7 |
| ISBN (Print) | 978-1-61737-984-0 |
| DOIs | |
| Publication status | Published - 2011 |
| MoE publication type | A3 Part of a book or another research book |
Keywords
- Lipidomics
- liquid chromatography
- mass spectrometry
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Nygren, H., Seppänen-Laakso, T., Castillo, S., Hyötyläinen, T., & Oresic, M. (2011). Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues. In T. O. Metz (Ed.), Metabolic Profiling: Methods and Protocols (pp. 247-257). Springer. https://doi.org/10.1007/978-1-61737-985-7_15