Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Orešič (Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

    113 Citations (Scopus)


    In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
    Original languageEnglish
    Title of host publicationMetabolic Profiling
    Subtitle of host publicationMethods and Protocols
    EditorsThomas O. Metz
    ISBN (Electronic)978-1-61737-985-7
    ISBN (Print)978-1-61737-984-0
    Publication statusPublished - 2011
    MoE publication typeA3 Part of a book or another research book

    Publication series

    SeriesMethods in Molecular Biology


    • Lipidomics
    • liquid chromatography
    • mass spectrometry


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