Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Orešič (Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

    96 Citations (Scopus)

    Abstract

    In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
    Original languageEnglish
    Title of host publicationMetabolic Profiling
    Subtitle of host publicationMethods and Protocols
    EditorsThomas O. Metz
    PublisherSpringer
    Chapter15
    Pages247-257
    ISBN (Electronic)978-1-61737-985-7
    ISBN (Print)978-1-61737-984-0
    DOIs
    Publication statusPublished - 2011
    MoE publication typeA3 Part of a book or another research book

    Publication series

    SeriesMethods in Molecular Biology
    Volume708
    ISSN1064-3745

    Keywords

    • Lipidomics
    • liquid chromatography
    • mass spectrometry

    Fingerprint

    Dive into the research topics of 'Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues'. Together they form a unique fingerprint.

    Cite this