Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Oresic

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

88 Citations (Scopus)

Abstract

In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS n mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
Original languageEnglish
Title of host publicationMetabolic Profiling
Subtitle of host publicationMethods and Protocols
EditorsThomas O. Metz
PublisherSpringer
Chapter15
Pages247-257
ISBN (Electronic)978-1-61737-985-7
ISBN (Print)978-1-61737-984-0
DOIs
Publication statusPublished - 2011
MoE publication typeA3 Part of a book or another research book

Keywords

  • Lipidomics
  • liquid chromatography
  • mass spectrometry

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    Nygren, H., Seppänen-Laakso, T., Castillo, S., Hyötyläinen, T., & Oresic, M. (2011). Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues. In T. O. Metz (Ed.), Metabolic Profiling: Methods and Protocols (pp. 247-257). Springer. https://doi.org/10.1007/978-1-61737-985-7_15