Localization of carboxypeptidase I in germinating barley grain

Harri Ranki, Tuomas Sopanen, Raimo Voutilainen

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Activity measurements and Northern blot hybridizations were used to study the temporal and spatial expression of carboxypeptidase I in germinating grains of barley (Hordeum vulgare L. cv Himalaya). In the resting grain no carboxypeptidase I activity was found in the aleurone layer, scutellum, or starchy endosperm.
During germination high levels of enzyme activity appeared in the scutellum and in the starchy endosperm but only low activity was found in the aleurone layer. No mRNA for carboxypeptidase I was observed in the resting grain. By day 1 of germination the mRNA appeared in the scutellum where its level remained high for several days.
In contrast, little mRNA was observed in the aleurone layer. These results indicate that the scutellum plays an important role in the production of carboxypeptidase I in germinating barley grain.
Original languageEnglish
Pages (from-to)1449-1452
JournalPlant Physiology
Volume93
Issue number4
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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