Low pH D-xylonate production with Pichia kudriavzevii

Mervi Toivari, Maija-Leena Vehkomäki, Yvonne Nygård, Merja Penttilä, Laura Ruohonen, Marilyn G. Wiebe

Research output: Contribution to journalArticleScientificpeer-review

76 Citations (Scopus)

Abstract

D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171g L-1 of D-xylonate from 171g L-1 d-xylose at a rate of 1.4g L-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146g L-1 D-xylonate at 1.2g L-1h-1 at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.

Original languageEnglish
Pages (from-to)555-562
JournalBioresource Technology
Volume133
DOIs
Publication statusPublished - 1 Jan 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • D-Xylonate
  • D-Xylose
  • Issatchenkia orientalis
  • Low pH
  • Pichia kudriavzevii

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