Low pH D-xylonate production with Pichia kudriavzevii

Mervi Toivari; Maija-Leena Vehkomäki; Yvonne Nygård; Merja Penttilä; Laura Ruohonen; Marilyn G. Wiebe

doi:10.1016/j.biortech.2013.01.157

Low pH D-xylonate production with Pichia kudriavzevii

Mervi Toivari^*, Maija-Leena Vehkomäki, Yvonne Nygård, Merja Penttilä, Laura Ruohonen, Marilyn G. Wiebe

^*Corresponding author for this work

BA53 Industrial biotechnology and food

VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

81 Citations (Scopus)

Abstract

D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171g L^-1 of D-xylonate from 171g L^-1 d-xylose at a rate of 1.4g L^-1h^-1 and yield of 1.0g [g substrate consumed]^-1, which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146g L^-1 D-xylonate at 1.2g L^-1h^-1 at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.

Original language	English
Pages (from-to)	555-562
Journal	Bioresource Technology
Volume	133
DOIs	https://doi.org/10.1016/j.biortech.2013.01.157
Publication status	Published - 1 Jan 2013
MoE publication type	A1 Journal article-refereed

Funding

This work has been financially supported by the Academy of Finland through the Centre of Excellence in White Biotechnology – Green Chemistry (Grant 118573 ) and the European Commission through the Seventh Framework Programme (FP7/2007-2013) under Grant agreement N° FP7-241566 BIOCORE. Financial support from the VTT Graduate School is acknowledged (Yvonne Nygård).

Keywords

D-Xylonate
D-Xylose
Issatchenkia orientalis
Low pH
Pichia kudriavzevii

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10.1016/j.biortech.2013.01.157Licence: CC BY-NC-ND

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title = "Low pH D-xylonate production with Pichia kudriavzevii",

abstract = "D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171g L-1 of D-xylonate from 171g L-1 d-xylose at a rate of 1.4g L-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146g L-1 D-xylonate at 1.2g L-1h-1 at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.",

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AU - Toivari, Mervi

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AU - Nygård, Yvonne

AU - Penttilä, Merja

AU - Ruohonen, Laura

AU - Wiebe, Marilyn G.

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N2 - D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171g L-1 of D-xylonate from 171g L-1 d-xylose at a rate of 1.4g L-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146g L-1 D-xylonate at 1.2g L-1h-1 at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.

AB - D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171g L-1 of D-xylonate from 171g L-1 d-xylose at a rate of 1.4g L-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146g L-1 D-xylonate at 1.2g L-1h-1 at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.

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KW - D-Xylose

KW - Issatchenkia orientalis

KW - Low pH

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ER -

Low pH D-xylonate production with Pichia kudriavzevii

Abstract

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Keywords

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