Abstract
d-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for d-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a d-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171gL-1 of d-xylonate from 171gL-1 d-xylose at a rate of 1.4gL-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with d-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146gL-1 d-xylonate at 1.2gL-1h-1 at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production.
Original language | English |
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Pages (from-to) | 555-562 |
Journal | Bioresource Technology |
Volume | 133 |
DOIs | |
Publication status | Published - 1 Jan 2013 |
MoE publication type | A1 Journal article-refereed |
Keywords
- D-Xylonate
- D-Xylose
- Issatchenkia orientalis
- Low pH
- Pichia kudriavzevii