Low pH d-xylonate production with Pichia kudriavzevii

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Abstract

d-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for d-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a d-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171gL-1 of d-xylonate from 171gL-1 d-xylose at a rate of 1.4gL-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with d-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146gL-1 d-xylonate at 1.2gL-1h-1 at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production.

Original languageEnglish
Pages (from-to)555-562
JournalBioresource Technology
Volume133
DOIs
Publication statusPublished - 1 Jan 2013
MoE publication typeA1 Journal article-refereed

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Keywords

  • D-Xylonate
  • D-Xylose
  • Issatchenkia orientalis
  • Low pH
  • Pichia kudriavzevii

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