Abstract
d-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for d-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a d-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171gL-1 of d-xylonate from 171gL-1 d-xylose at a rate of 1.4gL-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with d-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146gL-1 d-xylonate at 1.2gL-1h-1 at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production.
| Original language | English |
|---|---|
| Pages (from-to) | 555-562 |
| Journal | Bioresource Technology |
| Volume | 133 |
| DOIs | |
| Publication status | Published - 1 Jan 2013 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- D-Xylonate
- D-Xylose
- Issatchenkia orientalis
- Low pH
- Pichia kudriavzevii
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Low pH d-xylonate production with Pichia kudriavzevii. / Toivari, Mervi; Vehkomäki, Maija Leena; Nygård, Yvonne; Penttilä, Merja; Ruohonen, Laura; Wiebe, Marilyn G.
In: Bioresource Technology, Vol. 133, 01.01.2013, p. 555-562.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Low pH d-xylonate production with Pichia kudriavzevii
AU - Toivari, Mervi
AU - Vehkomäki, Maija Leena
AU - Nygård, Yvonne
AU - Penttilä, Merja
AU - Ruohonen, Laura
AU - Wiebe, Marilyn G.
N1 - CA2: TK402 CA2: TK400 SDA: BIC ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY
PY - 2013/1/1
Y1 - 2013/1/1
N2 - d-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for d-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a d-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171gL-1 of d-xylonate from 171gL-1 d-xylose at a rate of 1.4gL-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with d-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146gL-1 d-xylonate at 1.2gL-1h-1 at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production.
AB - d-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for d-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a d-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171gL-1 of d-xylonate from 171gL-1 d-xylose at a rate of 1.4gL-1h-1 and yield of 1.0g [g substrate consumed]-1, which was comparable with d-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146gL-1 d-xylonate at 1.2gL-1h-1 at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production.
KW - D-Xylonate
KW - D-Xylose
KW - Issatchenkia orientalis
KW - Low pH
KW - Pichia kudriavzevii
UR - http://www.scopus.com/inward/record.url?scp=84874547939&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2013.01.157
DO - 10.1016/j.biortech.2013.01.157
M3 - Article
VL - 133
SP - 555
EP - 562
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -