Maintenance of the anaerobic beer spoilage bacteria Pectinatus and Megasphaera

Maija-Liisa Suihko (Corresponding Author), Auli Haikara

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)


The preservation of Pectinatus and Megasphaera strains by freeze-drying and freezing, using different working methods and protective agents, was studied.Pectinatus strains isolated from beer, and all theMegasphaera strains, recovered well after freeze-drying and after freezing in reducing conditions. However, the recovery ofPectinatus strains isolated from yeast cultures was, in most of the conditions tested, very low, and after 2 years storage, they were almost non-viable. The only satisfactory method for these strains was rapid freezing in liquid nitrogen using 5% DMSO as protective agent.
Original languageEnglish
Pages (from-to)33-41
JournalFood Microbiology
Issue number1
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed


  • Pectinatus


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