Malt sprout extract medium for cultivation of Lactobacillus plantarum protective cultures

Arja Laitila (Corresponding Author), Maria Saarela, Liza Kirk, Matti Siika-aho, Auli Haikara, Tiina Mattila-Sandholm, Ilkka Virkajärvi

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Aims: The aim was to develop a cheap cereal‐based alternative medium for the large‐scale production of biopreservative Lactobacillus plantarum VTT E‐79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E‐79098 and the antimicrobial activity of the cell‐free extracts.

Methods: Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell‐free extracts against Pantoea agglomerans VTT E‐90396 and Fusarium avenaceum VTT D‐80147 was assessed with automated turbidometry.

Significance and Impact of the Study: When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E‐79098 and enhanced antimicrobial potential against the Gram‐negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low‐cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.

Original languageEnglish
Pages (from-to)336 - 340
Number of pages5
JournalLetters in Applied Microbiology
Volume39
Issue number4
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • antimicrobial
  • biocontrol
  • cultivation
  • growth medium
  • malt sprouts

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