Abstract
Aims: The aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative Lactobacillus plantarum VTT E-79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E-79098 and the antimicrobial activity of the cell‐free extracts.
Methods: Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell-free extracts against Pantoea agglomerans VTT E-90396 and Fusarium avenaceum VTT D-80147 was assessed with automated turbidometry.
Significance and Impact of the Study: When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E-79098 and enhanced antimicrobial potential against the Gram-negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low‐cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.
| Original language | English |
|---|---|
| Pages (from-to) | 336-340 |
| Journal | Letters in Applied Microbiology |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- antimicrobial
- biocontrol
- cultivation
- growth medium
- malt sprouts
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