Maltose and maltotriose utilisation by group I strains of the hybrid lager yeast Saccharomyces pastorianus

Frederico Magalhães (Corresponding Author), Virve Vidgren, Laura Ruohonen, Brian Gibson

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Brewer's wort is a challenging environment for yeast as it contains predominantly a-glucoside sugars. There exist two subgroups of the lager yeast Saccharomyces pastorianus which differ in sugar utilisation. We performed wort fermentations and compared representative strains from both groups with respect to their ability to transport and ferment maltose and maltotriose. Additionally, we mapped the transporters MALx1, AGT1, MPHx and MTT1 by Southern blotting. Contrary to previous observations, group I comprises a diverse set of strains, with varying ability to transport and ferment maltotriose. Of the eight group I strains, three efficiently utilised maltotriose, a property enabled by the presence of transmembrane transporters SeAGT1 and MTT1. A58, a variant of the group I type strain (CBS1513) performed particularly well, taking up maltotriose at a higher rate than maltose and retaining significant transport activity at temperatures as low as 0?C. Analysis of transporter distribution in this strain revealed an increased copy number of the MTT1 gene, which encodes the only permease known with higher affinity for maltotriose than maltose and low temperature dependence for transport. We propose that much of the variation in lager yeast fermentation behaviour is determined by the presence or absence of specific transmembrane transporters.
Original languageEnglish
Article numberfow053
JournalFEMS Yeast Research
Volume16
Issue number5
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Saccharomyces
Maltose
Yeasts
Fermentation
Temperature
Gene Dosage
Membrane Transport Proteins
Glucosides
Southern Blotting
maltotriose

Keywords

  • A-glucoside transporters
  • Group I
  • Hybrid
  • Lager
  • Maltotriose
  • MTT1

Cite this

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title = "Maltose and maltotriose utilisation by group I strains of the hybrid lager yeast Saccharomyces pastorianus",
abstract = "Brewer's wort is a challenging environment for yeast as it contains predominantly a-glucoside sugars. There exist two subgroups of the lager yeast Saccharomyces pastorianus which differ in sugar utilisation. We performed wort fermentations and compared representative strains from both groups with respect to their ability to transport and ferment maltose and maltotriose. Additionally, we mapped the transporters MALx1, AGT1, MPHx and MTT1 by Southern blotting. Contrary to previous observations, group I comprises a diverse set of strains, with varying ability to transport and ferment maltotriose. Of the eight group I strains, three efficiently utilised maltotriose, a property enabled by the presence of transmembrane transporters SeAGT1 and MTT1. A58, a variant of the group I type strain (CBS1513) performed particularly well, taking up maltotriose at a higher rate than maltose and retaining significant transport activity at temperatures as low as 0?C. Analysis of transporter distribution in this strain revealed an increased copy number of the MTT1 gene, which encodes the only permease known with higher affinity for maltotriose than maltose and low temperature dependence for transport. We propose that much of the variation in lager yeast fermentation behaviour is determined by the presence or absence of specific transmembrane transporters.",
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Maltose and maltotriose utilisation by group I strains of the hybrid lager yeast Saccharomyces pastorianus. / Magalhães, Frederico (Corresponding Author); Vidgren, Virve; Ruohonen, Laura; Gibson, Brian.

In: FEMS Yeast Research, Vol. 16, No. 5, fow053, 2016.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Gibson, Brian

PY - 2016

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