Abstract
The yeast Saccharomyces cerevisiae
has two closely related plasma membrane syntaxins, Sso1p and Sso2p,
which together provide an essential function in vegetative cells.
However, Sso1p is also specifically needed during sporulation; and this
function cannot be provided by Sso2p. We used fusions between SSO1 and SSO2 to map the sporulation-specific function of SSO1.
We found that the two N-terminal α-helices Ha and Hb of Sso1p are
important for sporulation, since it is reduced 8-fold for fusions where
Ha and Hb are derived from Sso2p. In contrast, the C-terminal half of
Sso1p does not seem to be specifically required for sporulation.
Surprisingly, we further found that the 3′ untranslated region (3′UTR)
of SSO1 is essential for
sporulation. Western blots failed to reveal a preferential expression of
Sso1p in sporulating cells, indicating that effects on gene expression
are unlikely to explain why the SSO1 3′UTR is needed for sporulation.
Original language | English |
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Pages (from-to) | 76 - 82 |
Number of pages | 7 |
Journal | Current Genetics |
Volume | 45 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Sso1p
- Sso2p
- Sporulation
- Syntaxin
- 3'Untranslated region
- yeasts