Mapping of sporulation-specific functions in the yeast syntaxin gene SSO1

Mattias Öyen, Jussi Jäntti, Sirkka Keränen, Hans Ronne (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The yeast Saccharomyces cerevisiae has two closely related plasma membrane syntaxins, Sso1p and Sso2p, which together provide an essential function in vegetative cells. However, Sso1p is also specifically needed during sporulation; and this function cannot be provided by Sso2p. We used fusions between SSO1 and SSO2 to map the sporulation-specific function of SSO1. We found that the two N-terminal α-helices Ha and Hb of Sso1p are important for sporulation, since it is reduced 8-fold for fusions where Ha and Hb are derived from Sso2p. In contrast, the C-terminal half of Sso1p does not seem to be specifically required for sporulation. Surprisingly, we further found that the 3′ untranslated region (3′UTR) of SSO1 is essential for sporulation. Western blots failed to reveal a preferential expression of Sso1p in sporulating cells, indicating that effects on gene expression are unlikely to explain why the SSO1 3′UTR is needed for sporulation.
Original languageEnglish
Pages (from-to)76 - 82
Number of pages7
JournalCurrent Genetics
Volume45
Issue number2
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Qa-SNARE Proteins
3' Untranslated Regions
Yeasts
Genes
Saccharomyces cerevisiae
Western Blotting
Cell Membrane
Gene Expression

Keywords

  • Sso1p
  • Sso2p
  • Sporulation
  • Syntaxin
  • 3'Untranslated region
  • yeasts

Cite this

Öyen, Mattias ; Jäntti, Jussi ; Keränen, Sirkka ; Ronne, Hans. / Mapping of sporulation-specific functions in the yeast syntaxin gene SSO1. In: Current Genetics. 2004 ; Vol. 45, No. 2. pp. 76 - 82.
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Mapping of sporulation-specific functions in the yeast syntaxin gene SSO1. / Öyen, Mattias; Jäntti, Jussi; Keränen, Sirkka; Ronne, Hans (Corresponding Author).

In: Current Genetics, Vol. 45, No. 2, 2004, p. 76 - 82.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Mapping of sporulation-specific functions in the yeast syntaxin gene SSO1

AU - Öyen, Mattias

AU - Jäntti, Jussi

AU - Keränen, Sirkka

AU - Ronne, Hans

PY - 2004

Y1 - 2004

N2 - The yeast Saccharomyces cerevisiae has two closely related plasma membrane syntaxins, Sso1p and Sso2p, which together provide an essential function in vegetative cells. However, Sso1p is also specifically needed during sporulation; and this function cannot be provided by Sso2p. We used fusions between SSO1 and SSO2 to map the sporulation-specific function of SSO1. We found that the two N-terminal α-helices Ha and Hb of Sso1p are important for sporulation, since it is reduced 8-fold for fusions where Ha and Hb are derived from Sso2p. In contrast, the C-terminal half of Sso1p does not seem to be specifically required for sporulation. Surprisingly, we further found that the 3′ untranslated region (3′UTR) of SSO1 is essential for sporulation. Western blots failed to reveal a preferential expression of Sso1p in sporulating cells, indicating that effects on gene expression are unlikely to explain why the SSO1 3′UTR is needed for sporulation.

AB - The yeast Saccharomyces cerevisiae has two closely related plasma membrane syntaxins, Sso1p and Sso2p, which together provide an essential function in vegetative cells. However, Sso1p is also specifically needed during sporulation; and this function cannot be provided by Sso2p. We used fusions between SSO1 and SSO2 to map the sporulation-specific function of SSO1. We found that the two N-terminal α-helices Ha and Hb of Sso1p are important for sporulation, since it is reduced 8-fold for fusions where Ha and Hb are derived from Sso2p. In contrast, the C-terminal half of Sso1p does not seem to be specifically required for sporulation. Surprisingly, we further found that the 3′ untranslated region (3′UTR) of SSO1 is essential for sporulation. Western blots failed to reveal a preferential expression of Sso1p in sporulating cells, indicating that effects on gene expression are unlikely to explain why the SSO1 3′UTR is needed for sporulation.

KW - Sso1p

KW - Sso2p

KW - Sporulation

KW - Syntaxin

KW - 3'Untranslated region

KW - yeasts

U2 - 10.1007/s00294-003-0462-8

DO - 10.1007/s00294-003-0462-8

M3 - Article

VL - 45

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EP - 82

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

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