Mapping The Properties and Performance of Nanocellulose- and Alginate-Based Cell Factory Matrices for Sustainable Photosynthetic Chemicals Production

Ville Rissanen (Corresponding author), Vilja Siitonen, Tuukka Levä, Lauri Nikkanen, Sergey Kosourov, Maria Heilala, Josphat Phiri, Thaddeus Maloney, Mikko Mäkelä, Yagut Allahverdiyeva, Tekla Tammelin

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Whole-cell immobilization in carrier matrices based on nanocellulosic hydrogels can be utilized in a range of application fields including biomedicine and biotechnology. One such emerging application is the so-called solid-state photosynthetic cell factory (SSFCF) aiming at sustainable biocatalytic chemicals production that contributes to circular bioeconomy. In the SSPCFs, immobilization has the potential to greatly improve the light-to-product ratio while reducing water and energy demands, but the efficiency of the system is integrally linked to the structure-property-performance interrelations of the nanocellulosic matrix. Thus, it is vital to employ interdisciplinary efforts based on materials, cell and bioprocess engineering to optimize the very distinct interplay between mechanical strength, mass transfer properties and biological performance of an efficient SSPCF. Here, we present a multidisciplinary experimental toolbox to investigate these properties from cross-linked matrix structures based on TEMPO-oxidized cellulose nanofibers (TCNF) and alginate. We use a combination of methods including rheology, multivariate data analyses, matrix structure investigations, and monitoring of gas exchange and biological operations of the immobilized photosynthetic cells. The obtained results give us new insight towards creating tailored cell factories – and immobilized systems in general – that can support the specific needs of the biological host and operational conditions.
Original languageEnglish
Title of host publicationNWBC 2022
Subtitle of host publicationThe 10th Nordic Wood Biorefinery Conference
EditorsAtte Virtanen, Katariina Torvinen, Jessica Vepsäläinen
PublisherVTT Technical Research Centre of Finland
Pages238-239
ISBN (Electronic)978-951-38-8772-8
Publication statusPublished - 2022
MoE publication typeNot Eligible
Event10th Nordic Wood Biorefinery Conference, NWBC 2022 - Helsinki, Finland
Duration: 25 Oct 202227 Oct 2022
Conference number: 10

Publication series

SeriesVTT Technology
Number409
ISSN2242-1211

Conference

Conference10th Nordic Wood Biorefinery Conference, NWBC 2022
Abbreviated titleNWBC 2022
Country/TerritoryFinland
CityHelsinki
Period25/10/2227/10/22

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