Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells

Toivo Naaranlahti, Seppo Lapinjoki, Aarre Huhtikangas, Leena Toivonen, Ulrika Kurten, Veli Kauppinen, Mauri Lounasmaa

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media.
When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells.
Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.
Original languageEnglish
Pages (from-to)155-157
JournalPlanta Medica
Volume55
Issue number2
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

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Catharanthus
Bony Callus
Alkaloids
Suspensions
High Pressure Liquid Chromatography
Assays
Screening
vindoline
Weights and Measures
Incidence

Cite this

Naaranlahti, T., Lapinjoki, S., Huhtikangas, A., Toivonen, L., Kurten, U., Kauppinen, V., & Lounasmaa, M. (1989). Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells. Planta Medica, 55(2), 155-157. https://doi.org/10.1055/s-2006-961911
Naaranlahti, Toivo ; Lapinjoki, Seppo ; Huhtikangas, Aarre ; Toivonen, Leena ; Kurten, Ulrika ; Kauppinen, Veli ; Lounasmaa, Mauri. / Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells. In: Planta Medica. 1989 ; Vol. 55, No. 2. pp. 155-157.
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abstract = "Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5){\%} dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.",
author = "Toivo Naaranlahti and Seppo Lapinjoki and Aarre Huhtikangas and Leena Toivonen and Ulrika Kurten and Veli Kauppinen and Mauri Lounasmaa",
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Naaranlahti, T, Lapinjoki, S, Huhtikangas, A, Toivonen, L, Kurten, U, Kauppinen, V & Lounasmaa, M 1989, 'Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells', Planta Medica, vol. 55, no. 2, pp. 155-157. https://doi.org/10.1055/s-2006-961911

Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells. / Naaranlahti, Toivo; Lapinjoki, Seppo; Huhtikangas, Aarre; Toivonen, Leena; Kurten, Ulrika; Kauppinen, Veli; Lounasmaa, Mauri.

In: Planta Medica, Vol. 55, No. 2, 1989, p. 155-157.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells

AU - Naaranlahti, Toivo

AU - Lapinjoki, Seppo

AU - Huhtikangas, Aarre

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AU - Kurten, Ulrika

AU - Kauppinen, Veli

AU - Lounasmaa, Mauri

PY - 1989

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N2 - Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.

AB - Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.

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Naaranlahti T, Lapinjoki S, Huhtikangas A, Toivonen L, Kurten U, Kauppinen V et al. Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells. Planta Medica. 1989;55(2):155-157. https://doi.org/10.1055/s-2006-961911