Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells

Toivo Naaranlahti, Seppo Lapinjoki, Aarre Huhtikangas, Leena Toivonen, Ulrika Kurten, Veli Kauppinen, Mauri Lounasmaa

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Abstract

Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media.
When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells.
Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.
Original languageEnglish
Pages (from-to)155-157
JournalPlanta Medica
Volume55
Issue number2
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

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    Naaranlahti, T., Lapinjoki, S., Huhtikangas, A., Toivonen, L., Kurten, U., Kauppinen, V., & Lounasmaa, M. (1989). Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells. Planta Medica, 55(2), 155-157. https://doi.org/10.1055/s-2006-961911