Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 33-39 |
| Number of pages | 7 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 2 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 1994 |
| MoE publication type | A1 Journal article-refereed |
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Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces. / Wirtanen, Gun (Corresponding Author); Mattila-Sandholm, Tiina.
In: Colloids and Surfaces B: Biointerfaces, Vol. 2, No. 1-3, 1994, p. 33-39.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces
AU - Wirtanen, Gun
AU - Mattila-Sandholm, Tiina
N1 - Project code: ELI2823
PY - 1994
Y1 - 1994
N2 - Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.
AB - Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.
U2 - 10.1016/0927-7765(94)80015-4
DO - 10.1016/0927-7765(94)80015-4
M3 - Article
VL - 2
SP - 33
EP - 39
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1-3
ER -