Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces

Gun Wirtanen (Corresponding Author), Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.
Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume2
Issue number1-3
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

Fingerprint

Pseudomonas fragi
biofilms
pseudomonas
Stainless Steel
Biofilms
image analysis
Image analysis
Glycocalyx
stainless steels
Stainless steel
Microscopy
Growth
plating
Plating
Microscopic examination
Steel
broths
microscopy
steels
Pediococcus pentosaceus

Cite this

@article{e66f268991af49f7a98e19e8f90a5cf1,
title = "Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces",
abstract = "Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.",
author = "Gun Wirtanen and Tiina Mattila-Sandholm",
note = "Project code: ELI2823",
year = "1994",
doi = "10.1016/0927-7765(94)80015-4",
language = "English",
volume = "2",
pages = "33--39",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",
number = "1-3",

}

Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces. / Wirtanen, Gun (Corresponding Author); Mattila-Sandholm, Tiina.

In: Colloids and Surfaces B: Biointerfaces, Vol. 2, No. 1-3, 1994, p. 33-39.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Measurement of biofilm of Pediococcus pentosaceus and Pseudomonas fragi on stainless steel surfaces

AU - Wirtanen, Gun

AU - Mattila-Sandholm, Tiina

N1 - Project code: ELI2823

PY - 1994

Y1 - 1994

N2 - Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.

AB - Biofilm was grown on stainless steel surfaces (AISI 304 and AISI 316) for 2, 5 and 10 days in slime broth inoculated with Pediococcus pentosaceus and Pseudomonas fragi. The steel surfaces on which biofilm had developed were investigated using conventional plating, quantitative glycocalyx determination and epifluorescence microscopy with image analysis. The results showed that after 10 days of growth the cells of P. fragi were difficult to cultivate from the surface and the growth was detected better by microscopy and image analysis. The slime produced by P. pentosaceus could be demonstrated by epifluorescence image analysis and quantitative glycocalyx measurement. The methods for detecting bacterial growth on surfaces are discussed with reference to conventional plating, image analysis and biofilm formation.

U2 - 10.1016/0927-7765(94)80015-4

DO - 10.1016/0927-7765(94)80015-4

M3 - Article

VL - 2

SP - 33

EP - 39

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 1-3

ER -