Measurement of xylanase activity with insoluble xylan substrate

Martti Nummi, Marja-Leena Niku-Paavola, Jean-Marc Perrin, Tor-Magnus Enari

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
Original languageEnglish
Pages (from-to)617-620
JournalBiochemical Journal
Volume226
Issue number2
Publication statusPublished - 1985
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylans
Betula
Hydrolysis
Substrates
Endo-1,4-beta Xylanases
Trichoderma
Aspergillus
Xylose
Alkalies
Sugars
Pulp
Assays
Ethanol
Acids
Enzymes
(1-4)-xylan

Cite this

Nummi, M., Niku-Paavola, M-L., Perrin, J-M., & Enari, T-M. (1985). Measurement of xylanase activity with insoluble xylan substrate. Biochemical Journal, 226(2), 617-620.
Nummi, Martti ; Niku-Paavola, Marja-Leena ; Perrin, Jean-Marc ; Enari, Tor-Magnus. / Measurement of xylanase activity with insoluble xylan substrate. In: Biochemical Journal. 1985 ; Vol. 226, No. 2. pp. 617-620.
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Nummi, M, Niku-Paavola, M-L, Perrin, J-M & Enari, T-M 1985, 'Measurement of xylanase activity with insoluble xylan substrate', Biochemical Journal, vol. 226, no. 2, pp. 617-620.

Measurement of xylanase activity with insoluble xylan substrate. / Nummi, Martti; Niku-Paavola, Marja-Leena; Perrin, Jean-Marc; Enari, Tor-Magnus.

In: Biochemical Journal, Vol. 226, No. 2, 1985, p. 617-620.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Measurement of xylanase activity with insoluble xylan substrate

AU - Nummi, Martti

AU - Niku-Paavola, Marja-Leena

AU - Perrin, Jean-Marc

AU - Enari, Tor-Magnus

PY - 1985

Y1 - 1985

N2 - Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

AB - Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

M3 - Article

VL - 226

SP - 617

EP - 620

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

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Nummi M, Niku-Paavola M-L, Perrin J-M, Enari T-M. Measurement of xylanase activity with insoluble xylan substrate. Biochemical Journal. 1985;226(2):617-620.