Measurement of xylanase activity with insoluble xylan substrate

Martti Nummi; Marja-Leena Niku-Paavola; Jean-Marc Perrin; Tor-Magnus Enari

doi:10.1042/bj2260617

Measurement of xylanase activity with insoluble xylan substrate

Martti Nummi, Marja-Leena Niku-Paavola, Jean-Marc Perrin, Tor-Magnus Enari

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

20 Citations (Scopus)

Abstract

Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

Original language	English
Pages (from-to)	617-620
Journal	Biochemical Journal
Volume	226
Issue number	2
DOIs	https://doi.org/10.1042/bj2260617
Publication status	Published - 1985
MoE publication type	A1 Journal article-refereed

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10.1042/bj2260617

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1144752/

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title = "Measurement of xylanase activity with insoluble xylan substrate",

abstract = "Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.",

author = "Martti Nummi and Marja-Leena Niku-Paavola and Jean-Marc Perrin and Tor-Magnus Enari",

year = "1985",

doi = "10.1042/bj2260617",

language = "English",

volume = "226",

pages = "617--620",

journal = "Biochemical Journal",

issn = "0264-6021",

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T1 - Measurement of xylanase activity with insoluble xylan substrate

AU - Nummi, Martti

AU - Niku-Paavola, Marja-Leena

AU - Perrin, Jean-Marc

AU - Enari, Tor-Magnus

PY - 1985

Y1 - 1985

N2 - Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

AB - Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

U2 - 10.1042/bj2260617

DO - 10.1042/bj2260617

M3 - Article

SN - 0264-6021

VL - 226

SP - 617

EP - 620

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Measurement of xylanase activity with insoluble xylan substrate

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