Measurement of xylanase activity with insoluble xylan substrate

Martti Nummi, Marja-Leena Niku-Paavola, Jean-Marc Perrin, Tor-Magnus Enari

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Abstract

Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
Original languageEnglish
Pages (from-to)617-620
JournalBiochemical Journal
Volume226
Issue number2
Publication statusPublished - 1985
MoE publication typeA1 Journal article-refereed

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Nummi, M., Niku-Paavola, M-L., Perrin, J-M., & Enari, T-M. (1985). Measurement of xylanase activity with insoluble xylan substrate. Biochemical Journal, 226(2), 617-620.