Measurements of fundamental properties of homogeneous tissue phantoms

Maciej S. Wróbel, Alexey P. Popov, Alexander V. Bykov, Matti Kinnunen, Malgorzata Jędrzejewska-Szczerska, Valery V. Tuchin

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)


We present the optical measurement techniques used in human skin phantom studies. Their accuracy and the sources of errors in microscopic parameters' estimation of the produced phantoms are described. We have produced optical phantoms for the purpose of simulating human skin tissue at the wavelength of 930 nm. Optical coherence tomography was used to measure the thickness and surface roughness and to detect the internal inhomogeneities. A more detailed study of phantom surface roughness was carried out with the optical profilometer. Reflectance, transmittance, and collimated transmittance of phantoms were measured using an integrating-sphere spectrometer setup. The scattering and absorption coefficients were calculated with the inverse adding-doubling method. The reduced scattering coefficient at 930 nm was found to be 1.57 ± 0.14 mm-1 and the absorption was 0.22 ± 0.03 mm-1. The retrieved optical properties of phantoms are in agreement with the data found in the literature for real human tissues.

Original languageEnglish
Article number045004
JournalJournal of Biomedical Optics
Issue number4
Publication statusPublished - 1 Apr 2015
MoE publication typeA1 Journal article-refereed


  • optical phantoms
  • optical properties
  • scattering
  • skin
  • spectrophotometry
  • tissue-mimicking phantoms


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