Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode

Maria Smolander (Corresponding Author), Hanna Livio, Lea Räsänen

    Research output: Contribution to journalArticleScientificpeer-review

    36 Citations (Scopus)

    Abstract

    An enzyme electrode was constructed for amperometric determination of xylose and glucose. The electrode is based on the PQQ-dependent membrane-bound aldose dehydrogenase (ALDH) from Gluconobacter oxydans. ALDH was covalently immobilized on a graphite electrode. Immobilized dimethylferrocene, soluble ferrocene carboxylic acid and phenazine metho-sulphate were used as electron transfer mediators. When xylose was measured electrochemically using an electrode modified with ALDH and dimethyl-ferrocene, the linear measurement range extended to 100 mM. For glucose measurement the linear measurement range was about one-tenth of that for xylose. The electrode showed fairly good stability: 50% of the original electrode response was still obtained after 5 days of intermittent use. The effect of possible leakage of adsorbed mediator was determined by measuring the response of an electrode with soluble mediator as a function of time. The reproducibility of the electrode was good, the standard deviation of the electrode response in ten measurements with the same electrode being only 2.7%.

    Original languageEnglish
    Pages (from-to)637-643
    JournalBiosensors & Bioelectronics
    Volume7
    Issue number9
    DOIs
    Publication statusPublished - 1992
    MoE publication typeA1 Journal article-refereed

    Keywords

    • biosensor
    • enzyme electrode
    • PQQ-dependent dehydrogenase
    • D-xylose
    • D-glucose

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