An enzyme electrode was constructed for amperometric determination of xylose and glucose. The electrode is based on the PQQ-dependent membrane-bound aldose dehydrogenase (ALDH) from Gluconobacter oxydans. ALDH was covalently immobilized on a graphite electrode. Immobilized dimethylferrocene, soluble ferrocene carboxylic acid and phenazine metho-sulphate were used as electron transfer mediators. When xylose was measured electrochemically using an electrode modified with ALDH and dimethyl-ferrocene, the linear measurement range extended to 100 mM. For glucose measurement the linear measurement range was about one-tenth of that for xylose. The electrode showed fairly good stability: 50% of the original electrode response was still obtained after 5 days of intermittent use. The effect of possible leakage of adsorbed mediator was determined by measuring the response of an electrode with soluble mediator as a function of time. The reproducibility of the electrode was good, the standard deviation of the electrode response in ten measurements with the same electrode being only 2.7%.
- enzyme electrode
- PQQ-dependent dehydrogenase
Smolander, M., Livio, H., & Räsänen, L. (1992). Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode. Biosensors & Bioelectronics, 7(9), 637-643. https://doi.org/10.1016/0956-5663(92)85021-2