Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode

Maria Smolander (Corresponding Author), Hanna Livio, Lea Räsänen

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

Abstract

An enzyme electrode was constructed for amperometric determination of xylose and glucose. The electrode is based on the PQQ-dependent membrane-bound aldose dehydrogenase (ALDH) from Gluconobacter oxydans. ALDH was covalently immobilized on a graphite electrode. Immobilized dimethylferrocene, soluble ferrocene carboxylic acid and phenazine metho-sulphate were used as electron transfer mediators. When xylose was measured electrochemically using an electrode modified with ALDH and dimethyl-ferrocene, the linear measurement range extended to 100 mM. For glucose measurement the linear measurement range was about one-tenth of that for xylose. The electrode showed fairly good stability: 50% of the original electrode response was still obtained after 5 days of intermittent use. The effect of possible leakage of adsorbed mediator was determined by measuring the response of an electrode with soluble mediator as a function of time. The reproducibility of the electrode was good, the standard deviation of the electrode response in ten measurements with the same electrode being only 2.7%.

Original languageEnglish
Pages (from-to)637-643
JournalBiosensors & Bioelectronics
Volume7
Issue number9
DOIs
Publication statusPublished - 1992
MoE publication typeA1 Journal article-refereed

Keywords

  • biosensor
  • enzyme electrode
  • PQQ-dependent dehydrogenase
  • D-xylose
  • D-glucose

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