Metabolic changes in serum metabolome in response to a meal

Aahana Shrestha, Elisabeth Müllner, Kaisa Poutanen, Hannu Mykkänen, Ali A. Moazzami

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Purpose: The change in serum metabolic response from fasting state to postprandial state provides novel insights into the impact of a single meal on human metabolism. Therefore, this study explored changes in serum metabolite profile after a single meal. Methods: Nineteen healthy postmenopausal women with normal glucose tolerance participated in the study. They received a meal consisting of refined wheat bread (50 g carbohydrates, 9 g protein, 4.2 g fat and 2.7 g dietary fibre), 40 g cucumber and 300 mL noncaloric orange drink. Blood samples were collected at fasting and five postprandial time points. Metabolic profile was measured by nuclear magnetic resonance and targeted liquid chromatography–mass spectrometry. Changes over time were assessed with multivariate models and ANOVA, with baseline as control. Results: The metabolomic analyses demonstrated alterations in phospholipids, amino acids and their breakdown products, glycolytic products, acylcarnitines and ketone bodies after a single meal. More specifically, phosphatidylcholines, lysophosphatidylcholines and citrate displayed an overall declining pattern, while leucine, isoleucine, methionine and succinate increased initially but declined thereafter. A sharp decline in acylcarnitines and ketone bodies and increase in glycolytic products postprandially suggest a switch in the body’s energy source from β-oxidation to glycolysis. Moreover, individuals with relatively high postprandial insulin responses generated a higher postprandial leucine responses compared to participants with lower insulin responses. Conclusions: The study demonstrated complex changes from catabolic to anabolic metabolism after a meal and indicated that the extent of postprandial responses is different between individuals with high and low insulin response.

Original languageEnglish
Pages (from-to)671-681
Number of pages11
JournalEuropean Journal of Nutrition
Volume56
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Metabolome
Meals
Ketone Bodies
Serum
Insulin
Leucine
Fasting
Cucumis sativus
Lysophosphatidylcholines
Metabolomics
Isoleucine
Bread
Dietary Fiber
Succinic Acid
Glycolysis
Phosphatidylcholines
Citric Acid
Methionine
Triticum
Phospholipids

Keywords

  • Acylcarnitine
  • Amino acid
  • Glycolytic products
  • Insulin
  • Metabolomics
  • Phosphatidylcholine
  • Postprandial changes

Cite this

Shrestha, Aahana ; Müllner, Elisabeth ; Poutanen, Kaisa ; Mykkänen, Hannu ; Moazzami, Ali A. / Metabolic changes in serum metabolome in response to a meal. In: European Journal of Nutrition. 2017 ; Vol. 56, No. 2. pp. 671-681.
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abstract = "Purpose: The change in serum metabolic response from fasting state to postprandial state provides novel insights into the impact of a single meal on human metabolism. Therefore, this study explored changes in serum metabolite profile after a single meal. Methods: Nineteen healthy postmenopausal women with normal glucose tolerance participated in the study. They received a meal consisting of refined wheat bread (50 g carbohydrates, 9 g protein, 4.2 g fat and 2.7 g dietary fibre), 40 g cucumber and 300 mL noncaloric orange drink. Blood samples were collected at fasting and five postprandial time points. Metabolic profile was measured by nuclear magnetic resonance and targeted liquid chromatography–mass spectrometry. Changes over time were assessed with multivariate models and ANOVA, with baseline as control. Results: The metabolomic analyses demonstrated alterations in phospholipids, amino acids and their breakdown products, glycolytic products, acylcarnitines and ketone bodies after a single meal. More specifically, phosphatidylcholines, lysophosphatidylcholines and citrate displayed an overall declining pattern, while leucine, isoleucine, methionine and succinate increased initially but declined thereafter. A sharp decline in acylcarnitines and ketone bodies and increase in glycolytic products postprandially suggest a switch in the body’s energy source from β-oxidation to glycolysis. Moreover, individuals with relatively high postprandial insulin responses generated a higher postprandial leucine responses compared to participants with lower insulin responses. Conclusions: The study demonstrated complex changes from catabolic to anabolic metabolism after a meal and indicated that the extent of postprandial responses is different between individuals with high and low insulin response.",
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Shrestha, A, Müllner, E, Poutanen, K, Mykkänen, H & Moazzami, AA 2017, 'Metabolic changes in serum metabolome in response to a meal', European Journal of Nutrition, vol. 56, no. 2, pp. 671-681. https://doi.org/10.1007/s00394-015-1111-y

Metabolic changes in serum metabolome in response to a meal. / Shrestha, Aahana; Müllner, Elisabeth; Poutanen, Kaisa; Mykkänen, Hannu; Moazzami, Ali A.

In: European Journal of Nutrition, Vol. 56, No. 2, 01.03.2017, p. 671-681.

Research output: Contribution to journalArticleScientificpeer-review

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