Abstract
An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.
| Original language | English |
|---|---|
| Pages (from-to) | 427-436 |
| Journal | Metabolic Engineering |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Jul 2012 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Bioconversion
- D-xylonic acid
- D-xylose
- D-xylose dehydrogenase
- Saccharomyces cerevisiae
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Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate. / Toivari, Mervi; Nygård, Yvonne; Kumpula, Esa Pekka; Vehkomäki, Maija Leena; Benčina, Mojca; Valkonen, Mari; Maaheimo, Hannu; Andberg, Martina; Koivula, Anu; Ruohonen, Laura; Penttilä, Merja; Wiebe, Marilyn G.
In: Metabolic Engineering, Vol. 14, No. 4, 01.07.2012, p. 427-436.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate
AU - Toivari, Mervi
AU - Nygård, Yvonne
AU - Kumpula, Esa Pekka
AU - Vehkomäki, Maija Leena
AU - Benčina, Mojca
AU - Valkonen, Mari
AU - Maaheimo, Hannu
AU - Andberg, Martina
AU - Koivula, Anu
AU - Ruohonen, Laura
AU - Penttilä, Merja
AU - Wiebe, Marilyn G.
N1 - CA2: TK402 CA2: TK404 CA2: TK400 SDA: BIC ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY
PY - 2012/7/1
Y1 - 2012/7/1
N2 - An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.
AB - An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.
KW - Bioconversion
KW - D-xylonic acid
KW - D-xylose
KW - D-xylose dehydrogenase
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=84862235495&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2012.03.002
DO - 10.1016/j.ymben.2012.03.002
M3 - Article
C2 - 22709678
AN - SCOPUS:84862235495
VL - 14
SP - 427
EP - 436
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
IS - 4
ER -