Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Mervi Toivari, Yvonne Nygård, Esa Pekka Kumpula, Maija Leena Vehkomäki, Mojca Benčina, Mari Valkonen, Hannu Maaheimo, Martina Andberg, Anu Koivula, Laura Ruohonen, Merja Penttilä, Marilyn G. Wiebe

    Research output: Contribution to journalArticleScientificpeer-review

    58 Citations (Scopus)

    Abstract

    An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.

    Original languageEnglish
    Pages (from-to)427-436
    JournalMetabolic Engineering
    Volume14
    Issue number4
    DOIs
    Publication statusPublished - 1 Jul 2012
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Bioconversion
    • D-xylonic acid
    • D-xylose
    • D-xylose dehydrogenase
    • Saccharomyces cerevisiae

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