Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Mervi Toivari; Yvonne Nygård; Esa Pekka Kumpula; Maija Leena Vehkomäki; Mojca Benčina; Mari Valkonen; Hannu Maaheimo; Martina Andberg; Anu Koivula; Laura Ruohonen; Merja Penttilä; Marilyn G. Wiebe

doi:10.1016/j.ymben.2012.03.002

Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Mervi Toivari^*
, Yvonne Nygård
, Esa Pekka Kumpula
, Maija Leena Vehkomäki
, Mojca Benčina
, Mari Valkonen
, Hannu Maaheimo
, Martina Andberg
, Anu Koivula
, Laura Ruohonen
, Merja Penttilä
, Marilyn G. Wiebe

^*Corresponding author for this work

BA53 Industrial biotechnology and food

VTT (former employee or external)
National Institute of Chemistry Ljubljana

Research output: Contribution to journal › Article › Scientific › peer-review

91 Citations (Scopus)

Abstract

An NAD ⁺-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l ^-1 at 0.23gl ^-1h ^-1 from 23g d-xylose l ^-1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP ⁺-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg ^-1; xylitol to ~18mgg ^-1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l ^-1 from 49g d-xylose l ^-1.

Original language	English
Pages (from-to)	427-436
Journal	Metabolic Engineering
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/j.ymben.2012.03.002
Publication status	Published - 1 Jul 2012
MoE publication type	A1 Journal article-refereed

Funding

Technical assistance of Jenni Kaija, Tarja Laakso, Outi Könönen and Tuuli Teikari is gratefully acknowledged. This study was financially supported by the Academy of Finland through the Centre of Excellence in White Biotechnology – Green Chemistry (grant 118573 ). The pH studies were funded with Academy of Finland researcher mobility grant ( 132169 ) and Slovenian Research Agency (grant BI-FI/11-12-019 ). Financial support from the VTT Graduate School is acknowledged (Yvonne Nygård). The financial support of the European Commission through the Sixth Framework Programme Integrated Project BioSynergy ( 038994-SES6 ) and the Seventh Framework Programme (FP7/2007-2013) under grant agreement No. FP7-241566 BIOCORE are also gratefully acknowledged.

Keywords

Bioconversion
D-xylonic acid
D-xylose
D-xylose dehydrogenase
Saccharomyces cerevisiae

Access to Document

10.1016/j.ymben.2012.03.002

Cite this

@article{85d099f0ed7448f1956641b4a891f991,

title = "Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate",

abstract = "An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to \textasciitilde{}70mgg -1; xylitol to \textasciitilde{}18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.",

keywords = "Bioconversion, D-xylonic acid, D-xylose, D-xylose dehydrogenase, Saccharomyces cerevisiae",

author = "Mervi Toivari and Yvonne Nyg{\aa}rd and Kumpula, \{Esa Pekka\} and Vehkom{\"a}ki, \{Maija Leena\} and Mojca Ben{\v c}ina and Mari Valkonen and Hannu Maaheimo and Martina Andberg and Anu Koivula and Laura Ruohonen and Merja Penttil{\"a} and Wiebe, \{Marilyn G.\}",

year = "2012",

month = jul,

day = "1",

doi = "10.1016/j.ymben.2012.03.002",

language = "English",

volume = "14",

pages = "427--436",

journal = "Metabolic Engineering",

issn = "1096-7176",

publisher = "Academic Press",

number = "4",

}

TY - JOUR

T1 - Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

AU - Toivari, Mervi

AU - Nygård, Yvonne

AU - Kumpula, Esa Pekka

AU - Vehkomäki, Maija Leena

AU - Benčina, Mojca

AU - Valkonen, Mari

AU - Maaheimo, Hannu

AU - Andberg, Martina

AU - Koivula, Anu

AU - Ruohonen, Laura

AU - Penttilä, Merja

AU - Wiebe, Marilyn G.

PY - 2012/7/1

Y1 - 2012/7/1

N2 - An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.

AB - An NAD +-dependent d-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting in production of 17±2g d-xylonate l -1 at 0.23gl -1h -1 from 23g d-xylose l -1 (with glucose and ethanol as co-substrates). d-Xylonate titre and production rate were increased and xylitol production decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP +-dependent d-xylose dehydrogenases. d-Xylonate accumulated intracellularly to ~70mgg -1; xylitol to ~18mgg -1. The aldose reductase encoding gene GRE3 was deleted to reduce xylitol production. Cells expressing d-xylonolactone lactonase xylC from C. crescentus with xylB initially produced more extracellular d-xylonate than cells lacking xylC at both pH 5.5 and pH 3, and sustained higher production at pH 3. Cell vitality and viability decreased during d-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43g d-xylonate l -1 from 49g d-xylose l -1.

KW - Bioconversion

KW - D-xylonic acid

KW - D-xylose

KW - D-xylose dehydrogenase

KW - Saccharomyces cerevisiae

UR - https://www.scopus.com/pages/publications/84862235495

U2 - 10.1016/j.ymben.2012.03.002

DO - 10.1016/j.ymben.2012.03.002

M3 - Article

C2 - 22709678

AN - SCOPUS:84862235495

SN - 1096-7176

VL - 14

SP - 427

EP - 436

JO - Metabolic Engineering

JF - Metabolic Engineering

IS - 4

ER -

Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this