Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols

Mervi H. Toivari, Laura Ruohonen, Andrei N. Miasnikov, Peter Richard, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

Original languageEnglish
Pages (from-to)5471-5476
JournalApplied and Environmental Microbiology
Volume73
Issue number17
DOIs
Publication statusPublished - 1 Sep 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Ribitol
Sugar Alcohols
Metabolic Engineering
Xylitol
sugar alcohols
xylitol
metabolic engineering
Transketolase
xylulose
transketolase
Saccharomyces cerevisiae
alcohol
sugar
glucose
Carbon
Xylulose
sugars
Pentoses
ribulose
Glucose

Cite this

@article{63f084d4361c47819f0261cd53893568,
title = "Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols",
abstract = "Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50{\%} of the 5-carbon sugar alcohols excreted.",
author = "Toivari, {Mervi H.} and Laura Ruohonen and Miasnikov, {Andrei N.} and Peter Richard and Merja Penttil{\"a}",
year = "2007",
month = "9",
day = "1",
doi = "10.1128/AEM.02707-06",
language = "English",
volume = "73",
pages = "5471--5476",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "17",

}

Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols. / Toivari, Mervi H.; Ruohonen, Laura; Miasnikov, Andrei N.; Richard, Peter; Penttilä, Merja.

In: Applied and Environmental Microbiology, Vol. 73, No. 17, 01.09.2007, p. 5471-5476.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols

AU - Toivari, Mervi H.

AU - Ruohonen, Laura

AU - Miasnikov, Andrei N.

AU - Richard, Peter

AU - Penttilä, Merja

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

AB - Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

UR - http://www.scopus.com/inward/record.url?scp=34548508967&partnerID=8YFLogxK

U2 - 10.1128/AEM.02707-06

DO - 10.1128/AEM.02707-06

M3 - Article

VL - 73

SP - 5471

EP - 5476

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 17

ER -