Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols

Mervi H. Toivari, Laura Ruohonen, Andrei N. Miasnikov, Peter Richard, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

Original languageEnglish
Pages (from-to)5471-5476
JournalApplied and Environmental Microbiology
Volume73
Issue number17
DOIs
Publication statusPublished - 1 Sep 2007
MoE publication typeA1 Journal article-refereed

Fingerprint Dive into the research topics of 'Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols'. Together they form a unique fingerprint.

  • Cite this