Abstract
New methods for an extended physiological characterization of yeast at a
microtiter plate scale were applied to 27 deletion mutants of Saccharomyces
cerevisiae cultivated on glucose and galactose as sole carbon sources. In this
way, specific growth rates, specific rates of glucose consumption and ethanol
production were determined. Flux distribution, particularly concerning
branching into the pentose phosphate pathway was determined using a new
13C-labelling method using MALDI-ToF-mass spectrometry. On glucose, the growth
was predominantly fermentative whereas on galactose respiration was more
active with correspondingly lower ethanol production. Some deletion strains
showed unexpected behavior providing very informative data about the function
of the corresponding gene. Deletion of malic enzyme gene, MAE1, did not show
any significant phenotype when grown on glucose but a drastically increased
branching from glucose 6-phosphate into the pentose phosphate pathway when
grown on galactose. This allows the conclusion that MAE1 is important for the
supply of NADPH during aerobic growth on galactose.
Original language | English |
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Pages (from-to) | 395-404 |
Journal | Journal of Biotechnology |
Volume | 132 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2007 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Saccharomyces cerevisiae
- Malic enzyme
- Microtiter plate cultivation
- MALDI-ToF-mass spectrometry
- Phenotypic analysis