Metabolie engineering of fungal strains for conversion of D-galacturonate to meso-Galactarate

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47 Citations (Scopus)

Abstract

D-Galacturonic acid can be obtained by hydrolyzing pectin, which is an abundant and low value raw material. By means of metabolic engineering, we constructed fungal strains for the conversion of D-galacturonate to meso-galactarate (mucate). Galactarate has applications in food, cosmetics, and pharmaceuticals and as a platform chemical. In fungi D-galacturonate is catabolized through a reductive pathway with a D-galacturonate reductase as the first enzyme. Deleting the corresponding gene in the fungi Hypocrea jecorina and Aspergillus niger resulted in strains unable to grow on D-galacturonate. The genes of the pathway for D-galacturonate catabolism were upregulated in the presence of D-galacturonate in A. niger, even when the gene for Dgalacturonate reductase was deleted, indicating that D-galacturonate itself is an inducer for the pathway. A bacterial gene coding for a D-galacturonate dehydrogenase catalyzing the NAD-dependent oxidation of Dgalacturonate to galactarate was introduced to both strains with disrupted D-galacturonate catabolism. Both strains converted D-galacturonate to galactarate. The resulting H. jecorina strain produced galactarate at high yield. The A. niger strain regained the ability to grow on D-galacturonate when the D-galacturonate dehydrogenase was introduced, suggesting that it has a pathway for galactarate catabolism.

Original languageEnglish
Pages (from-to)169-175
JournalApplied and Environmental Microbiology
Volume76
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010
MoE publication typeA1 Journal article-refereed

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Aspergillus niger
catabolism
Oxidoreductases
engineering
gene
Hypocrea
Fungi
Genes
Metabolic Engineering
Bacterial Genes
Trichoderma reesei
fungus
Cosmetics
NAD
metabolism
genes
Food
galacturonic acid
metabolic engineering
fungi

Cite this

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title = "Metabolie engineering of fungal strains for conversion of D-galacturonate to meso-Galactarate",
abstract = "D-Galacturonic acid can be obtained by hydrolyzing pectin, which is an abundant and low value raw material. By means of metabolic engineering, we constructed fungal strains for the conversion of D-galacturonate to meso-galactarate (mucate). Galactarate has applications in food, cosmetics, and pharmaceuticals and as a platform chemical. In fungi D-galacturonate is catabolized through a reductive pathway with a D-galacturonate reductase as the first enzyme. Deleting the corresponding gene in the fungi Hypocrea jecorina and Aspergillus niger resulted in strains unable to grow on D-galacturonate. The genes of the pathway for D-galacturonate catabolism were upregulated in the presence of D-galacturonate in A. niger, even when the gene for Dgalacturonate reductase was deleted, indicating that D-galacturonate itself is an inducer for the pathway. A bacterial gene coding for a D-galacturonate dehydrogenase catalyzing the NAD-dependent oxidation of Dgalacturonate to galactarate was introduced to both strains with disrupted D-galacturonate catabolism. Both strains converted D-galacturonate to galactarate. The resulting H. jecorina strain produced galactarate at high yield. The A. niger strain regained the ability to grow on D-galacturonate when the D-galacturonate dehydrogenase was introduced, suggesting that it has a pathway for galactarate catabolism.",
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Metabolie engineering of fungal strains for conversion of D-galacturonate to meso-Galactarate. / Mojzita, Dominik; Wiebe, Marilyn; Hilditch, Satu; Boer, Harry; Penttilä, Merja; Richard, Peter.

In: Applied and Environmental Microbiology, Vol. 76, No. 1, 01.01.2010, p. 169-175.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Wiebe, Marilyn

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