Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine

Umidbek S. Akbarov (Corresponding Author), Olga N. Pozharitskaya, Into Laakso, Tuulikki Seppänen-Laakso, Irina N. Urakova, Heikki Vuorela, Valery G. Makarov, Alexander N. Shikov

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Ethnopharmacological relevance: Aqueous autolysate from the snake Eryx miliaris (SNA) has been used in traditional medicine of Uzbekistan as anti-inflammatory, hepatoprotective and immunomodulatory agent. However, little is known about the chemical composition and its mechanisms of activity. Aim of the study: This is our first attempt to analyse the composition of snake autolysate using gas chromatography with mass spectrometry (GC-MS) and to investigate the mechanisms of anti-inflammatory and hyaluronidase activity of fingerprinted E. miliaris autolysate to support their use in the traditional Uzbek medicine. Materials and methods: Aqueous autolysate was evaporated and derivatised for GC-MS analysis of metabolites. For quantification, lipids were extracted from autolysate by solvent extraction and derivatised by esterification and silylation. Biological activity was evaluated with lipid peroxidation, cyclooxygenase (COX) inhibition and antihyaluronidase activity tests. Results: GC-MS analysis of SNA enabled the identification of 27 compounds. Short chain fatty acids (SCFA, 21%), amino acid/derivatives 39% (incl. 2-piperidinone 19%), phenyl (7%), and OH-Phenyl (10%) derivatives covered 77%. Other derivatives (9%) included succinic acid and 3-indole acetic acid). Long chain fatty acids (C16–C18) accounted for 3%. The lipid concentration of SNA was 1.2 mg/mL (0.12%). Three concentration levels (1.0–20.0 μg/mL) did not inhibit COX-1 and COX-2 in vitro and malondialdehyde level was not decreased by SNA in lipid peroxidation model. However, SNA was a potent inhibitor of the hyaluronidase enzyme activity in a dose dependent manner with IC50 = 0.086 mL/mL. Conclusion: The results from GC-MS analyses of SNA lead us to the identification of a wide range of major chemical structures of the metabolites and their derivatives with several categories. Pharmacological studies support the traditional use of SNA and show one of its possible mechanisms of activity via inhibition of hyaluronidase.

Original languageEnglish
Article number112459
JournalJournal of Ethnopharmacology
DOIs
Publication statusAccepted/In press - 4 Dec 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

Snakes
Traditional Medicine
Gas Chromatography-Mass Spectrometry
Hyaluronoglucosaminidase
Lipid Peroxidation
Anti-Inflammatory Agents
Uzbekistan
Lipids
Cyclooxygenase 1
Volatile Fatty Acids
Esterification
Succinic Acid
Enzyme Inhibitors
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Malondialdehyde
Inhibitory Concentration 50
Fatty Acids
Pharmacology
Amino Acids

Keywords

  • Anti-inflammatory activity
  • Eryx miliaris
  • Gas chromatography mass spectrometry
  • Hyaluronidase

Cite this

Akbarov, U. S., Pozharitskaya, O. N., Laakso, I., Seppänen-Laakso, T., Urakova, I. N., Vuorela, H., ... Shikov, A. N. (Accepted/In press). Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine. Journal of Ethnopharmacology, [112459]. https://doi.org/10.1016/j.jep.2019.112459
Akbarov, Umidbek S. ; Pozharitskaya, Olga N. ; Laakso, Into ; Seppänen-Laakso, Tuulikki ; Urakova, Irina N. ; Vuorela, Heikki ; Makarov, Valery G. ; Shikov, Alexander N. / Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine. In: Journal of Ethnopharmacology. 2019.
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title = "Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine",
abstract = "Ethnopharmacological relevance: Aqueous autolysate from the snake Eryx miliaris (SNA) has been used in traditional medicine of Uzbekistan as anti-inflammatory, hepatoprotective and immunomodulatory agent. However, little is known about the chemical composition and its mechanisms of activity. Aim of the study: This is our first attempt to analyse the composition of snake autolysate using gas chromatography with mass spectrometry (GC-MS) and to investigate the mechanisms of anti-inflammatory and hyaluronidase activity of fingerprinted E. miliaris autolysate to support their use in the traditional Uzbek medicine. Materials and methods: Aqueous autolysate was evaporated and derivatised for GC-MS analysis of metabolites. For quantification, lipids were extracted from autolysate by solvent extraction and derivatised by esterification and silylation. Biological activity was evaluated with lipid peroxidation, cyclooxygenase (COX) inhibition and antihyaluronidase activity tests. Results: GC-MS analysis of SNA enabled the identification of 27 compounds. Short chain fatty acids (SCFA, 21{\%}), amino acid/derivatives 39{\%} (incl. 2-piperidinone 19{\%}), phenyl (7{\%}), and OH-Phenyl (10{\%}) derivatives covered 77{\%}. Other derivatives (9{\%}) included succinic acid and 3-indole acetic acid). Long chain fatty acids (C16–C18) accounted for 3{\%}. The lipid concentration of SNA was 1.2 mg/mL (0.12{\%}). Three concentration levels (1.0–20.0 μg/mL) did not inhibit COX-1 and COX-2 in vitro and malondialdehyde level was not decreased by SNA in lipid peroxidation model. However, SNA was a potent inhibitor of the hyaluronidase enzyme activity in a dose dependent manner with IC50 = 0.086 mL/mL. Conclusion: The results from GC-MS analyses of SNA lead us to the identification of a wide range of major chemical structures of the metabolites and their derivatives with several categories. Pharmacological studies support the traditional use of SNA and show one of its possible mechanisms of activity via inhibition of hyaluronidase.",
keywords = "Anti-inflammatory activity, Eryx miliaris, Gas chromatography mass spectrometry, Hyaluronidase",
author = "Akbarov, {Umidbek S.} and Pozharitskaya, {Olga N.} and Into Laakso and Tuulikki Sepp{\"a}nen-Laakso and Urakova, {Irina N.} and Heikki Vuorela and Makarov, {Valery G.} and Shikov, {Alexander N.}",
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Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine. / Akbarov, Umidbek S. (Corresponding Author); Pozharitskaya, Olga N.; Laakso, Into; Seppänen-Laakso, Tuulikki; Urakova, Irina N.; Vuorela, Heikki; Makarov, Valery G.; Shikov, Alexander N.

In: Journal of Ethnopharmacology, 04.12.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Metabolite profiling and mechanisms of bioactivity of snake autolysate - A traditional Uzbek medicine

AU - Akbarov, Umidbek S.

AU - Pozharitskaya, Olga N.

AU - Laakso, Into

AU - Seppänen-Laakso, Tuulikki

AU - Urakova, Irina N.

AU - Vuorela, Heikki

AU - Makarov, Valery G.

AU - Shikov, Alexander N.

PY - 2019/12/4

Y1 - 2019/12/4

N2 - Ethnopharmacological relevance: Aqueous autolysate from the snake Eryx miliaris (SNA) has been used in traditional medicine of Uzbekistan as anti-inflammatory, hepatoprotective and immunomodulatory agent. However, little is known about the chemical composition and its mechanisms of activity. Aim of the study: This is our first attempt to analyse the composition of snake autolysate using gas chromatography with mass spectrometry (GC-MS) and to investigate the mechanisms of anti-inflammatory and hyaluronidase activity of fingerprinted E. miliaris autolysate to support their use in the traditional Uzbek medicine. Materials and methods: Aqueous autolysate was evaporated and derivatised for GC-MS analysis of metabolites. For quantification, lipids were extracted from autolysate by solvent extraction and derivatised by esterification and silylation. Biological activity was evaluated with lipid peroxidation, cyclooxygenase (COX) inhibition and antihyaluronidase activity tests. Results: GC-MS analysis of SNA enabled the identification of 27 compounds. Short chain fatty acids (SCFA, 21%), amino acid/derivatives 39% (incl. 2-piperidinone 19%), phenyl (7%), and OH-Phenyl (10%) derivatives covered 77%. Other derivatives (9%) included succinic acid and 3-indole acetic acid). Long chain fatty acids (C16–C18) accounted for 3%. The lipid concentration of SNA was 1.2 mg/mL (0.12%). Three concentration levels (1.0–20.0 μg/mL) did not inhibit COX-1 and COX-2 in vitro and malondialdehyde level was not decreased by SNA in lipid peroxidation model. However, SNA was a potent inhibitor of the hyaluronidase enzyme activity in a dose dependent manner with IC50 = 0.086 mL/mL. Conclusion: The results from GC-MS analyses of SNA lead us to the identification of a wide range of major chemical structures of the metabolites and their derivatives with several categories. Pharmacological studies support the traditional use of SNA and show one of its possible mechanisms of activity via inhibition of hyaluronidase.

AB - Ethnopharmacological relevance: Aqueous autolysate from the snake Eryx miliaris (SNA) has been used in traditional medicine of Uzbekistan as anti-inflammatory, hepatoprotective and immunomodulatory agent. However, little is known about the chemical composition and its mechanisms of activity. Aim of the study: This is our first attempt to analyse the composition of snake autolysate using gas chromatography with mass spectrometry (GC-MS) and to investigate the mechanisms of anti-inflammatory and hyaluronidase activity of fingerprinted E. miliaris autolysate to support their use in the traditional Uzbek medicine. Materials and methods: Aqueous autolysate was evaporated and derivatised for GC-MS analysis of metabolites. For quantification, lipids were extracted from autolysate by solvent extraction and derivatised by esterification and silylation. Biological activity was evaluated with lipid peroxidation, cyclooxygenase (COX) inhibition and antihyaluronidase activity tests. Results: GC-MS analysis of SNA enabled the identification of 27 compounds. Short chain fatty acids (SCFA, 21%), amino acid/derivatives 39% (incl. 2-piperidinone 19%), phenyl (7%), and OH-Phenyl (10%) derivatives covered 77%. Other derivatives (9%) included succinic acid and 3-indole acetic acid). Long chain fatty acids (C16–C18) accounted for 3%. The lipid concentration of SNA was 1.2 mg/mL (0.12%). Three concentration levels (1.0–20.0 μg/mL) did not inhibit COX-1 and COX-2 in vitro and malondialdehyde level was not decreased by SNA in lipid peroxidation model. However, SNA was a potent inhibitor of the hyaluronidase enzyme activity in a dose dependent manner with IC50 = 0.086 mL/mL. Conclusion: The results from GC-MS analyses of SNA lead us to the identification of a wide range of major chemical structures of the metabolites and their derivatives with several categories. Pharmacological studies support the traditional use of SNA and show one of its possible mechanisms of activity via inhibition of hyaluronidase.

KW - Anti-inflammatory activity

KW - Eryx miliaris

KW - Gas chromatography mass spectrometry

KW - Hyaluronidase

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