Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: A Big Mac challenge

I Bondia-Pons, Johanna Maukonen, Ismo Mattila, A Rissanen, Maria Saarela, J Kaprio, A Hakkarainen, J Lundbom, N Lundbom, T Hyötyläinen, K H Pietiläinen, M Oresic

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Postprandial responses to food are complex, involving both genetic and environmental factors. We studied postprandial responses to a Big Mac meal challenge in monozygotic co-twins highly discordant for body weight. This unique design allows assessment of the contribution of obesity, independent of genetic liability. Comprehensive metabolic profiling using 3 analytical platforms was applied to fasting and postprandial serum samples from 16 healthy monozygotic twin pairs discordant for weight (body mass index difference >3 kg/m2). Nine concordant monozygotic pairs were examined as control pairs. Fecal samples were analyzed to assess diversity of the major bacterial groups by using 5 different validated bacterial group specific denaturing gradient gel electrophoresis methods. No differences in fecal bacterial diversity were detected when comparing co-twins discordant for weight (ANOVA, P
Original languageEnglish
Pages (from-to)4169-4179
Number of pages10
JournalFASEB Journal
Volume28
Issue number9
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Metabolome
Monozygotic Twins
Microbiota
Analysis of variance (ANOVA)
Electrophoresis
Gels
Weights and Measures
Denaturing Gradient Gel Electrophoresis
Meals
Fasting
Analysis of Variance
Body Mass Index
Obesity
Body Weight
Food
Serum

Keywords

  • Obesity
  • metabolomics
  • bile acids

Cite this

Bondia-Pons, I., Maukonen, J., Mattila, I., Rissanen, A., Saarela, M., Kaprio, J., ... Oresic, M. (2014). Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: A Big Mac challenge. FASEB Journal, 28(9), 4169-4179. https://doi.org/10.1096/fj.14-250167
Bondia-Pons, I ; Maukonen, Johanna ; Mattila, Ismo ; Rissanen, A ; Saarela, Maria ; Kaprio, J ; Hakkarainen, A ; Lundbom, J ; Lundbom, N ; Hyötyläinen, T ; Pietiläinen, K H ; Oresic, M. / Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight : A Big Mac challenge. In: FASEB Journal. 2014 ; Vol. 28, No. 9. pp. 4169-4179.
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Bondia-Pons, I, Maukonen, J, Mattila, I, Rissanen, A, Saarela, M, Kaprio, J, Hakkarainen, A, Lundbom, J, Lundbom, N, Hyötyläinen, T, Pietiläinen, KH & Oresic, M 2014, 'Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: A Big Mac challenge', FASEB Journal, vol. 28, no. 9, pp. 4169-4179. https://doi.org/10.1096/fj.14-250167

Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight : A Big Mac challenge. / Bondia-Pons, I; Maukonen, Johanna; Mattila, Ismo; Rissanen, A; Saarela, Maria; Kaprio, J; Hakkarainen, A; Lundbom, J; Lundbom, N; Hyötyläinen, T; Pietiläinen, K H; Oresic, M.

In: FASEB Journal, Vol. 28, No. 9, 2014, p. 4169-4179.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Maukonen, Johanna

AU - Mattila, Ismo

AU - Rissanen, A

AU - Saarela, Maria

AU - Kaprio, J

AU - Hakkarainen, A

AU - Lundbom, J

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AU - Hyötyläinen, T

AU - Pietiläinen, K H

AU - Oresic, M

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Bondia-Pons I, Maukonen J, Mattila I, Rissanen A, Saarela M, Kaprio J et al. Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: A Big Mac challenge. FASEB Journal. 2014;28(9):4169-4179. https://doi.org/10.1096/fj.14-250167