Metabolomic analysis of polar metabolites in lipoprotein fractions identifies lipoprotein-specific metabolic profiles and their association with insulin resistance

Tuulia Hyötyläinen (Corresponding Author), Ismo Mattila, Susanne K. Wiedmer, Artturi Koivuniemi, Marja-Riitta Taskinen, Hannele Yki-Järvinen, Matej Orešič

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

While the molecular lipid composition of lipoproteins has been investigated in detail, little is known about associations of small polar metabolites with specific lipoproteins. The aim of the present study was to investigate the profiles of polar metabolites in different lipoprotein fractions, i.e., very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL) and two sub-fractions of the high-density lipoprotein (HDL). The VLDL, IDL, LDL, HDL2, and HDL3 fractions were isolated from serum of sixteen individuals having a broad range of insulin sensitivity and characterized using comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOFMS). The lipoprotein fractions had clearly different metabolite profiles, which correlated with the particle size and surface charge. Lipoprotein-specific associations of individual metabolites with insulin resistance were identified, particularly in VLDL and IDL fractions, even in the absence of such associations in serum. The results indicate that the polar molecules are strongly attached to the surface of the lipoproteins. Furthermore, strong lipoprotein-specific associations of metabolites with insulin resistance, as compared to their serum profiles, indicate that lipoproteins may be a rich source of tissue-specific metabolic biomarkers.
Original languageEnglish
Pages (from-to)2559-2565
JournalMolecular bioSystems
Volume8
Issue number10
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Metabolomics
Metabolome
Lipoproteins
Insulin Resistance
IDL Lipoproteins
VLDL Lipoproteins
LDL Lipoproteins
Serum
HDL Lipoproteins
Particle Size
Gas Chromatography
Mass Spectrometry
Biomarkers
Lipids

Keywords

  • Lipoproteins
  • GCxGC-TOFMS
  • VLDL
  • IDL
  • LDL
  • HDL2
  • HDL3
  • metabolomics
  • metabolic syndrome
  • insulin resistance

Cite this

Hyötyläinen, Tuulia ; Mattila, Ismo ; Wiedmer, Susanne K. ; Koivuniemi, Artturi ; Taskinen, Marja-Riitta ; Yki-Järvinen, Hannele ; Orešič, Matej. / Metabolomic analysis of polar metabolites in lipoprotein fractions identifies lipoprotein-specific metabolic profiles and their association with insulin resistance. In: Molecular bioSystems. 2012 ; Vol. 8, No. 10. pp. 2559-2565.
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abstract = "While the molecular lipid composition of lipoproteins has been investigated in detail, little is known about associations of small polar metabolites with specific lipoproteins. The aim of the present study was to investigate the profiles of polar metabolites in different lipoprotein fractions, i.e., very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL) and two sub-fractions of the high-density lipoprotein (HDL). The VLDL, IDL, LDL, HDL2, and HDL3 fractions were isolated from serum of sixteen individuals having a broad range of insulin sensitivity and characterized using comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOFMS). The lipoprotein fractions had clearly different metabolite profiles, which correlated with the particle size and surface charge. Lipoprotein-specific associations of individual metabolites with insulin resistance were identified, particularly in VLDL and IDL fractions, even in the absence of such associations in serum. The results indicate that the polar molecules are strongly attached to the surface of the lipoproteins. Furthermore, strong lipoprotein-specific associations of metabolites with insulin resistance, as compared to their serum profiles, indicate that lipoproteins may be a rich source of tissue-specific metabolic biomarkers.",
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author = "Tuulia Hy{\"o}tyl{\"a}inen and Ismo Mattila and Wiedmer, {Susanne K.} and Artturi Koivuniemi and Marja-Riitta Taskinen and Hannele Yki-J{\"a}rvinen and Matej Orešič",
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Hyötyläinen, T, Mattila, I, Wiedmer, SK, Koivuniemi, A, Taskinen, M-R, Yki-Järvinen, H & Orešič, M 2012, 'Metabolomic analysis of polar metabolites in lipoprotein fractions identifies lipoprotein-specific metabolic profiles and their association with insulin resistance', Molecular bioSystems, vol. 8, no. 10, pp. 2559-2565. https://doi.org/10.1039/C2MB25115A

Metabolomic analysis of polar metabolites in lipoprotein fractions identifies lipoprotein-specific metabolic profiles and their association with insulin resistance. / Hyötyläinen, Tuulia (Corresponding Author); Mattila, Ismo; Wiedmer, Susanne K.; Koivuniemi, Artturi; Taskinen, Marja-Riitta; Yki-Järvinen, Hannele; Orešič, Matej.

In: Molecular bioSystems, Vol. 8, No. 10, 2012, p. 2559-2565.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Metabolomic analysis of polar metabolites in lipoprotein fractions identifies lipoprotein-specific metabolic profiles and their association with insulin resistance

AU - Hyötyläinen, Tuulia

AU - Mattila, Ismo

AU - Wiedmer, Susanne K.

AU - Koivuniemi, Artturi

AU - Taskinen, Marja-Riitta

AU - Yki-Järvinen, Hannele

AU - Orešič, Matej

PY - 2012

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N2 - While the molecular lipid composition of lipoproteins has been investigated in detail, little is known about associations of small polar metabolites with specific lipoproteins. The aim of the present study was to investigate the profiles of polar metabolites in different lipoprotein fractions, i.e., very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL) and two sub-fractions of the high-density lipoprotein (HDL). The VLDL, IDL, LDL, HDL2, and HDL3 fractions were isolated from serum of sixteen individuals having a broad range of insulin sensitivity and characterized using comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOFMS). The lipoprotein fractions had clearly different metabolite profiles, which correlated with the particle size and surface charge. Lipoprotein-specific associations of individual metabolites with insulin resistance were identified, particularly in VLDL and IDL fractions, even in the absence of such associations in serum. The results indicate that the polar molecules are strongly attached to the surface of the lipoproteins. Furthermore, strong lipoprotein-specific associations of metabolites with insulin resistance, as compared to their serum profiles, indicate that lipoproteins may be a rich source of tissue-specific metabolic biomarkers.

AB - While the molecular lipid composition of lipoproteins has been investigated in detail, little is known about associations of small polar metabolites with specific lipoproteins. The aim of the present study was to investigate the profiles of polar metabolites in different lipoprotein fractions, i.e., very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL) and two sub-fractions of the high-density lipoprotein (HDL). The VLDL, IDL, LDL, HDL2, and HDL3 fractions were isolated from serum of sixteen individuals having a broad range of insulin sensitivity and characterized using comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOFMS). The lipoprotein fractions had clearly different metabolite profiles, which correlated with the particle size and surface charge. Lipoprotein-specific associations of individual metabolites with insulin resistance were identified, particularly in VLDL and IDL fractions, even in the absence of such associations in serum. The results indicate that the polar molecules are strongly attached to the surface of the lipoproteins. Furthermore, strong lipoprotein-specific associations of metabolites with insulin resistance, as compared to their serum profiles, indicate that lipoproteins may be a rich source of tissue-specific metabolic biomarkers.

KW - Lipoproteins

KW - GCxGC-TOFMS

KW - VLDL

KW - IDL

KW - LDL

KW - HDL2

KW - HDL3

KW - metabolomics

KW - metabolic syndrome

KW - insulin resistance

U2 - 10.1039/C2MB25115A

DO - 10.1039/C2MB25115A

M3 - Article

VL - 8

SP - 2559

EP - 2565

JO - Molecular bioSystems

JF - Molecular bioSystems

SN - 1742-206X

IS - 10

ER -