Method and test kit for quantitative determination of polynucleotides in a mixture

Hans Soederlund; Reetta Satokari; Kari Kataja; Kristiina Takkinen

Method and test kit for quantitative determination of polynucleotides in a mixture

Hans Soederlund (Inventor), Reetta Satokari (Inventor), Kari Kataja (Inventor), Kristiina Takkinen (Inventor)

BA6306 Photonic biosensor concepts

Research output: Patent › Patent

Abstract

The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.

Patent family as of 26.8.2021
AT384141 E 20080215 AT20030762698T 20030704
AU2003242804 AA 20040123 AU20030242804 20030704
AU2003242804 BB 20080612 AU20030242804 20030704
CA2489196 AA 20041209 CA20032489196 20030704
CA2489196 C 20130611 CA20032489196 20030704
DE60318723 D1 20080306 DE20036018723T 20030704
DE60318723 T2 20090115 DE20036018723T 20030704
DK1537238 T3 20080602 DK20030762698T 20030704
EP1537238 A1 20050608 EP20030762698 20030704
EP1537238 B1 20080116 EP20030762698 20030704
EP1947200 A1 20080723 EP20080100037 20030704
ES2299727 T3 20080601 ES20030762698T 20030704
FI20021325 A0 20020705 FI20020001325 20020705
JP2005532058 T2 20051027 JP20040518823T 20030704
JP4672365 B2 20110420 JP20040518823T 20030704
PT1537238 T 20080422 PT20030762698T 20030704
SI1537238 T1 20080630 SI20030031180T 20030704
US2006035228 AA 20060216 US20030519403 20030704
US7361461 BB 20080422 US20030519403 20030704
US2008233591 AA 20080925 US20080105960 20080418
US8143388 BB 20120327 US20080105960 20080418
WO04005545 A1 20040115 WO2003FI00544 20030704

Link to current patent family on right

Original language	English
Patent number	EP1537238
IPC	C12Q 1/ 6816 A I
Priority date	4/07/03
Publication status	Published - 8 Jun 2005
MoE publication type	H1 Granted patent

Cite this

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title = "Method and test kit for quantitative determination of polynucleotides in a mixture",

abstract = "The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.Patent family as of 26.8.2021 AT384141 E 20080215 AT20030762698T 20030704 AU2003242804 AA 20040123 AU20030242804 20030704 AU2003242804 BB 20080612 AU20030242804 20030704 CA2489196 AA 20041209 CA20032489196 20030704 CA2489196 C 20130611 CA20032489196 20030704 DE60318723 D1 20080306 DE20036018723T 20030704 DE60318723 T2 20090115 DE20036018723T 20030704 DK1537238 T3 20080602 DK20030762698T 20030704 EP1537238 A1 20050608 EP20030762698 20030704 EP1537238 B1 20080116 EP20030762698 20030704 EP1947200 A1 20080723 EP20080100037 20030704 ES2299727 T3 20080601 ES20030762698T 20030704 FI20021325 A0 20020705 FI20020001325 20020705 JP2005532058 T2 20051027 JP20040518823T 20030704 JP4672365 B2 20110420 JP20040518823T 20030704 PT1537238 T 20080422 PT20030762698T 20030704 SI1537238 T1 20080630 SI20030031180T 20030704 US2006035228 AA 20060216 US20030519403 20030704 US7361461 BB 20080422 US20030519403 20030704 US2008233591 AA 20080925 US20080105960 20080418 US8143388 BB 20120327 US20080105960 20080418 WO04005545 A1 20040115 WO2003FI00544 20030704Link to current patent family on right ",

author = "Hans Soederlund and Reetta Satokari and Kari Kataja and Kristiina Takkinen",

year = "2005",

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day = "8",

language = "English",

type = "Patent",

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TY - PAT

T1 - Method and test kit for quantitative determination of polynucleotides in a mixture

AU - Soederlund, Hans

AU - Satokari, Reetta

AU - Kataja, Kari

AU - Takkinen, Kristiina

PY - 2005/6/8

Y1 - 2005/6/8

N2 - The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.Patent family as of 26.8.2021 AT384141 E 20080215 AT20030762698T 20030704 AU2003242804 AA 20040123 AU20030242804 20030704 AU2003242804 BB 20080612 AU20030242804 20030704 CA2489196 AA 20041209 CA20032489196 20030704 CA2489196 C 20130611 CA20032489196 20030704 DE60318723 D1 20080306 DE20036018723T 20030704 DE60318723 T2 20090115 DE20036018723T 20030704 DK1537238 T3 20080602 DK20030762698T 20030704 EP1537238 A1 20050608 EP20030762698 20030704 EP1537238 B1 20080116 EP20030762698 20030704 EP1947200 A1 20080723 EP20080100037 20030704 ES2299727 T3 20080601 ES20030762698T 20030704 FI20021325 A0 20020705 FI20020001325 20020705 JP2005532058 T2 20051027 JP20040518823T 20030704 JP4672365 B2 20110420 JP20040518823T 20030704 PT1537238 T 20080422 PT20030762698T 20030704 SI1537238 T1 20080630 SI20030031180T 20030704 US2006035228 AA 20060216 US20030519403 20030704 US7361461 BB 20080422 US20030519403 20030704 US2008233591 AA 20080925 US20080105960 20080418 US8143388 BB 20120327 US20080105960 20080418 WO04005545 A1 20040115 WO2003FI00544 20030704Link to current patent family on right

AB - The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.Patent family as of 26.8.2021 AT384141 E 20080215 AT20030762698T 20030704 AU2003242804 AA 20040123 AU20030242804 20030704 AU2003242804 BB 20080612 AU20030242804 20030704 CA2489196 AA 20041209 CA20032489196 20030704 CA2489196 C 20130611 CA20032489196 20030704 DE60318723 D1 20080306 DE20036018723T 20030704 DE60318723 T2 20090115 DE20036018723T 20030704 DK1537238 T3 20080602 DK20030762698T 20030704 EP1537238 A1 20050608 EP20030762698 20030704 EP1537238 B1 20080116 EP20030762698 20030704 EP1947200 A1 20080723 EP20080100037 20030704 ES2299727 T3 20080601 ES20030762698T 20030704 FI20021325 A0 20020705 FI20020001325 20020705 JP2005532058 T2 20051027 JP20040518823T 20030704 JP4672365 B2 20110420 JP20040518823T 20030704 PT1537238 T 20080422 PT20030762698T 20030704 SI1537238 T1 20080630 SI20030031180T 20030704 US2006035228 AA 20060216 US20030519403 20030704 US7361461 BB 20080422 US20030519403 20030704 US2008233591 AA 20080925 US20080105960 20080418 US8143388 BB 20120327 US20080105960 20080418 WO04005545 A1 20040115 WO2003FI00544 20030704Link to current patent family on right

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M3 - Patent

M1 - EP1537238

ER -

Method and test kit for quantitative determination of polynucleotides in a mixture

Abstract

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