Methods for identifying lipoxygenase producing microorganisms on agar plates

Antti Nyyssölä, R. Heshof, T. Haarmann, J. Eidner, Ann Westerholm-Parvinen, K. Langfelder, Kristiina Kruus, L. de Graaff (Corresponding Author), Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Plate assays for lipoxygenase producing microorganisms on agar plates have been developed. Both potassium iodide-starch and indamine dye formation methods were effective for detecting soybean lipoxygenase activity on agar plates. A positive result was also achieved using the β-carotene bleaching method, but the sensitivity of this method was lower than the other two methods. The potassium iodide-starch and indamine dye formation methods were also applied for detecting lipoxygenase production by Trichoderma reesei and Pichia pastoris transformants expressing the lipoxygenase gene of the fungus Gaeumannomyces graminis. In both cases lipoxygenase production in the transformants could be identified. For detection of the G. graminis lipoxygenase produced by Aspergillus nidulans the potassium iodide-starch method was successful. When Escherichia coli was grown on agar and soybean lipoxygenase was applied on the culture lipoxygenase activity could clearly be detected by the indamine dye formation method. This suggests that the method has potential for screening of metagenomic libraries in E. coli for lipoxygenase activity.
Original languageEnglish
Number of pages7
JournalAMB Express
Volume2
Issue number17
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Lipoxygenase
Agar
Potassium Iodide
Starch
Coloring Agents
Soybeans
Escherichia coli
Metagenomics
Aspergillus nidulans
Trichoderma
Pichia
Carotenoids
Fungi

Cite this

Nyyssölä, Antti ; Heshof, R. ; Haarmann, T. ; Eidner, J. ; Westerholm-Parvinen, Ann ; Langfelder, K. ; Kruus, Kristiina ; de Graaff, L. ; Buchert, Johanna. / Methods for identifying lipoxygenase producing microorganisms on agar plates. In: AMB Express. 2012 ; Vol. 2, No. 17.
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title = "Methods for identifying lipoxygenase producing microorganisms on agar plates",
abstract = "Plate assays for lipoxygenase producing microorganisms on agar plates have been developed. Both potassium iodide-starch and indamine dye formation methods were effective for detecting soybean lipoxygenase activity on agar plates. A positive result was also achieved using the β-carotene bleaching method, but the sensitivity of this method was lower than the other two methods. The potassium iodide-starch and indamine dye formation methods were also applied for detecting lipoxygenase production by Trichoderma reesei and Pichia pastoris transformants expressing the lipoxygenase gene of the fungus Gaeumannomyces graminis. In both cases lipoxygenase production in the transformants could be identified. For detection of the G. graminis lipoxygenase produced by Aspergillus nidulans the potassium iodide-starch method was successful. When Escherichia coli was grown on agar and soybean lipoxygenase was applied on the culture lipoxygenase activity could clearly be detected by the indamine dye formation method. This suggests that the method has potential for screening of metagenomic libraries in E. coli for lipoxygenase activity.",
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Methods for identifying lipoxygenase producing microorganisms on agar plates. / Nyyssölä, Antti; Heshof, R.; Haarmann, T.; Eidner, J.; Westerholm-Parvinen, Ann; Langfelder, K.; Kruus, Kristiina; de Graaff, L. (Corresponding Author); Buchert, Johanna.

In: AMB Express, Vol. 2, No. 17, 2012.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Methods for identifying lipoxygenase producing microorganisms on agar plates

AU - Nyyssölä, Antti

AU - Heshof, R.

AU - Haarmann, T.

AU - Eidner, J.

AU - Westerholm-Parvinen, Ann

AU - Langfelder, K.

AU - Kruus, Kristiina

AU - de Graaff, L.

AU - Buchert, Johanna

PY - 2012

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AB - Plate assays for lipoxygenase producing microorganisms on agar plates have been developed. Both potassium iodide-starch and indamine dye formation methods were effective for detecting soybean lipoxygenase activity on agar plates. A positive result was also achieved using the β-carotene bleaching method, but the sensitivity of this method was lower than the other two methods. The potassium iodide-starch and indamine dye formation methods were also applied for detecting lipoxygenase production by Trichoderma reesei and Pichia pastoris transformants expressing the lipoxygenase gene of the fungus Gaeumannomyces graminis. In both cases lipoxygenase production in the transformants could be identified. For detection of the G. graminis lipoxygenase produced by Aspergillus nidulans the potassium iodide-starch method was successful. When Escherichia coli was grown on agar and soybean lipoxygenase was applied on the culture lipoxygenase activity could clearly be detected by the indamine dye formation method. This suggests that the method has potential for screening of metagenomic libraries in E. coli for lipoxygenase activity.

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