Abstract
Multitiered quantitative analysis of biological systems is rapidly
becoming the desired approach to study hierarchical functional
interactions between proteins and metabolites. We describe here a novel
systematic approach to analyze organisms with complex metabolic
regulatory networks. By using precise analytical methods to measure
biochemical constituents and their relative abundance in whole plasma of
transgenic ApoE*3-Leiden mice and an isogenic wild-type control group,
simultaneous snapshots of metabolic and protein states were obtained.
Novel data processing and multivariate analysis tools such as Impurity
Resolution Software (IMPRESS™) and Windows-based linear fit program
(WINLIN™) were used to compare protein and metabolic profiles in
parallel. Canonical correlations of the resulting data show quantitative
relationships between heterogeneous components in the TG animals. These
results, obtained solely from whole plasma analysis allowed us, in a
rapid manner, to corroborate previous findings as well as find new
events pertaining to dominant and peripheral events in lipoprotein
metabolism of a genetically modified mammalian organism in relation to
ApoE3, a key mediator of lipoprotein metabolism.
Original language | English |
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Pages (from-to) | 267-288 |
Journal | OMICS: A Journal of Integrative Biology |
Volume | 8 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2004 |
MoE publication type | A1 Journal article-refereed |