Microbial attachment and biofilm formation in brewery bottling plants

Fijación Microbiana y Formación de Biofilm en Plantas de Embotellamiento de Cervecerías

Erna Storgårds (Corresponding Author), Kaisa Tapani, Peter Hartwall, Riitta Saleva, Maija-Liisa Suihko

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

Microbial attachment and biofilm formation in brewery bottling plants was studied by mounting sterile, stainless steel coupons onto critical sites of the fillers in three breweries. Microbiological samples were taken from the coupons to reveal the primary colonizers in biofilm formation. The bacteria were identified using ribotyping, carbohydrate fermentation tests, and partial 16S rDNA sequencing. The effect of sugars and sweeteners on the attachment of primary colonizers to stainless steel was analyzed with epifluorescence microscopy. The biofilm formation rate was studied for eight weeks by successively dislodging the test coupons from each sampling site and examining them with epifluorescence microscopy. The results showed that primary colonizers accumulated on new stainless steel surfaces within hours after the start of production. Regular daily cleaning reduced the number of microorganisms on the surfaces only momentarily. Canning machines were markedly less prone to accumulation of microorganisms than bottling machines. Gram-negative bacteria, yeasts, and molds were the first to colonize the surfaces. Attachment of primary colonizers to stainless steel was increased by sugars and sweeteners. Horizontal surfaces were prone to microbial accumulation and should be avoided in constructions as much as possible. Furthermore, biofilm formation occurred on certain surfaces despite daily cleaning and disinfection.
Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalJournal of the American Society of Brewing Chemists
Volume64
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

bottling
brewing industry
Stainless Steel
Biofilms
biofilm
stainless steel
Sweetening Agents
sweeteners
Microscopy
cleaning
microscopy
Ribotyping
fillers (equipment)
Disinfection
sugars
microorganisms
Ribosomal DNA
Gram-Negative Bacteria
canning
Fermentation

Keywords

  • hygiene
  • packaging
  • primary colonizers
  • sugars
  • sweeteners

Cite this

@article{272dcf4dc3fa4576a29929ded0904f46,
title = "Microbial attachment and biofilm formation in brewery bottling plants: Fijaci{\'o}n Microbiana y Formaci{\'o}n de Biofilm en Plantas de Embotellamiento de Cervecer{\'i}as",
abstract = "Microbial attachment and biofilm formation in brewery bottling plants was studied by mounting sterile, stainless steel coupons onto critical sites of the fillers in three breweries. Microbiological samples were taken from the coupons to reveal the primary colonizers in biofilm formation. The bacteria were identified using ribotyping, carbohydrate fermentation tests, and partial 16S rDNA sequencing. The effect of sugars and sweeteners on the attachment of primary colonizers to stainless steel was analyzed with epifluorescence microscopy. The biofilm formation rate was studied for eight weeks by successively dislodging the test coupons from each sampling site and examining them with epifluorescence microscopy. The results showed that primary colonizers accumulated on new stainless steel surfaces within hours after the start of production. Regular daily cleaning reduced the number of microorganisms on the surfaces only momentarily. Canning machines were markedly less prone to accumulation of microorganisms than bottling machines. Gram-negative bacteria, yeasts, and molds were the first to colonize the surfaces. Attachment of primary colonizers to stainless steel was increased by sugars and sweeteners. Horizontal surfaces were prone to microbial accumulation and should be avoided in constructions as much as possible. Furthermore, biofilm formation occurred on certain surfaces despite daily cleaning and disinfection.",
keywords = "hygiene, packaging, primary colonizers, sugars, sweeteners",
author = "Erna Storg{\aa}rds and Kaisa Tapani and Peter Hartwall and Riitta Saleva and Maija-Liisa Suihko",
year = "2006",
doi = "10.1094/ASBCJ-64-0008",
language = "English",
volume = "64",
pages = "8--15",
journal = "Journal of the American Society of Brewing Chemists",
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Microbial attachment and biofilm formation in brewery bottling plants : Fijación Microbiana y Formación de Biofilm en Plantas de Embotellamiento de Cervecerías. / Storgårds, Erna (Corresponding Author); Tapani, Kaisa; Hartwall, Peter; Saleva, Riitta; Suihko, Maija-Liisa.

In: Journal of the American Society of Brewing Chemists, Vol. 64, No. 1, 2006, p. 8-15.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Microbial attachment and biofilm formation in brewery bottling plants

T2 - Fijación Microbiana y Formación de Biofilm en Plantas de Embotellamiento de Cervecerías

AU - Storgårds, Erna

AU - Tapani, Kaisa

AU - Hartwall, Peter

AU - Saleva, Riitta

AU - Suihko, Maija-Liisa

PY - 2006

Y1 - 2006

N2 - Microbial attachment and biofilm formation in brewery bottling plants was studied by mounting sterile, stainless steel coupons onto critical sites of the fillers in three breweries. Microbiological samples were taken from the coupons to reveal the primary colonizers in biofilm formation. The bacteria were identified using ribotyping, carbohydrate fermentation tests, and partial 16S rDNA sequencing. The effect of sugars and sweeteners on the attachment of primary colonizers to stainless steel was analyzed with epifluorescence microscopy. The biofilm formation rate was studied for eight weeks by successively dislodging the test coupons from each sampling site and examining them with epifluorescence microscopy. The results showed that primary colonizers accumulated on new stainless steel surfaces within hours after the start of production. Regular daily cleaning reduced the number of microorganisms on the surfaces only momentarily. Canning machines were markedly less prone to accumulation of microorganisms than bottling machines. Gram-negative bacteria, yeasts, and molds were the first to colonize the surfaces. Attachment of primary colonizers to stainless steel was increased by sugars and sweeteners. Horizontal surfaces were prone to microbial accumulation and should be avoided in constructions as much as possible. Furthermore, biofilm formation occurred on certain surfaces despite daily cleaning and disinfection.

AB - Microbial attachment and biofilm formation in brewery bottling plants was studied by mounting sterile, stainless steel coupons onto critical sites of the fillers in three breweries. Microbiological samples were taken from the coupons to reveal the primary colonizers in biofilm formation. The bacteria were identified using ribotyping, carbohydrate fermentation tests, and partial 16S rDNA sequencing. The effect of sugars and sweeteners on the attachment of primary colonizers to stainless steel was analyzed with epifluorescence microscopy. The biofilm formation rate was studied for eight weeks by successively dislodging the test coupons from each sampling site and examining them with epifluorescence microscopy. The results showed that primary colonizers accumulated on new stainless steel surfaces within hours after the start of production. Regular daily cleaning reduced the number of microorganisms on the surfaces only momentarily. Canning machines were markedly less prone to accumulation of microorganisms than bottling machines. Gram-negative bacteria, yeasts, and molds were the first to colonize the surfaces. Attachment of primary colonizers to stainless steel was increased by sugars and sweeteners. Horizontal surfaces were prone to microbial accumulation and should be avoided in constructions as much as possible. Furthermore, biofilm formation occurred on certain surfaces despite daily cleaning and disinfection.

KW - hygiene

KW - packaging

KW - primary colonizers

KW - sugars

KW - sweeteners

U2 - 10.1094/ASBCJ-64-0008

DO - 10.1094/ASBCJ-64-0008

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JF - Journal of the American Society of Brewing Chemists

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