Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems

Gun Wirtanen (Corresponding Author), Ulrika Husmark, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

84 Citations (Scopus)

Abstract

The biotransfer potential in food processing is defined as the ability of the microorganisms present on equipment surfaces both before and after cleaning procedures to contaminate products during processing. Growth of Bacillus biofilms was detectable on both stainless-steel and Teflon© surfaces after all the cleaning procedures. B. cereus and B. subtilis cells adhered more firmly to unsoiled than to soiled surfaces, whereas B. thuringiensis adhered most firmly to soiled surfaces. The results showed that the removal of biofilms from surfaces was more difficult from stainless steel because the microorganisms were attached more tenaciously to rough surfaces. Biofilms were cleaned most effectively from unsoiled surfaces with a simple rinsing procedure; thus the mechanical forces of the flow are very important in the cleaning. The chemical and heat treatments used for cleaning had the greatest impact on the abundance of living microorganisms. All the procedures tested did not remove biofilm material effectively from the surfaces. Significantly more cells were removed when the alkaline phase in the alkaline-acid treatment of the cleaning-inplace (CIP) procedure was prolonged, and ethylene diaminetetraacetic acid (EDTA) was used.
Original languageEnglish
Pages (from-to)727-733
Number of pages7
JournalJournal of Food Protection
Volume59
Issue number7
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Food Handling
Bacillus (bacteria)
Biofilms
food processing
cleaning
biofilm
Bacillus
Stainless Steel
stainless steel
microorganisms
Acids
Polytetrafluoroethylene
EDTA (chelating agent)
chemical treatment
Hot Temperature
Bacillus cereus
acid treatment
Bacillus subtilis
Equipment and Supplies
Growth

Cite this

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title = "Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems",
abstract = "The biotransfer potential in food processing is defined as the ability of the microorganisms present on equipment surfaces both before and after cleaning procedures to contaminate products during processing. Growth of Bacillus biofilms was detectable on both stainless-steel and Teflon{\circledC} surfaces after all the cleaning procedures. B. cereus and B. subtilis cells adhered more firmly to unsoiled than to soiled surfaces, whereas B. thuringiensis adhered most firmly to soiled surfaces. The results showed that the removal of biofilms from surfaces was more difficult from stainless steel because the microorganisms were attached more tenaciously to rough surfaces. Biofilms were cleaned most effectively from unsoiled surfaces with a simple rinsing procedure; thus the mechanical forces of the flow are very important in the cleaning. The chemical and heat treatments used for cleaning had the greatest impact on the abundance of living microorganisms. All the procedures tested did not remove biofilm material effectively from the surfaces. Significantly more cells were removed when the alkaline phase in the alkaline-acid treatment of the cleaning-inplace (CIP) procedure was prolonged, and ethylene diaminetetraacetic acid (EDTA) was used.",
author = "Gun Wirtanen and Ulrika Husmark and Tiina Mattila-Sandholm",
note = "Project code: B5SU00117",
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journal = "Journal of Food Protection",
issn = "0362-028X",
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Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems. / Wirtanen, Gun (Corresponding Author); Husmark, Ulrika; Mattila-Sandholm, Tiina.

In: Journal of Food Protection, Vol. 59, No. 7, 1996, p. 727-733.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems

AU - Wirtanen, Gun

AU - Husmark, Ulrika

AU - Mattila-Sandholm, Tiina

N1 - Project code: B5SU00117

PY - 1996

Y1 - 1996

N2 - The biotransfer potential in food processing is defined as the ability of the microorganisms present on equipment surfaces both before and after cleaning procedures to contaminate products during processing. Growth of Bacillus biofilms was detectable on both stainless-steel and Teflon© surfaces after all the cleaning procedures. B. cereus and B. subtilis cells adhered more firmly to unsoiled than to soiled surfaces, whereas B. thuringiensis adhered most firmly to soiled surfaces. The results showed that the removal of biofilms from surfaces was more difficult from stainless steel because the microorganisms were attached more tenaciously to rough surfaces. Biofilms were cleaned most effectively from unsoiled surfaces with a simple rinsing procedure; thus the mechanical forces of the flow are very important in the cleaning. The chemical and heat treatments used for cleaning had the greatest impact on the abundance of living microorganisms. All the procedures tested did not remove biofilm material effectively from the surfaces. Significantly more cells were removed when the alkaline phase in the alkaline-acid treatment of the cleaning-inplace (CIP) procedure was prolonged, and ethylene diaminetetraacetic acid (EDTA) was used.

AB - The biotransfer potential in food processing is defined as the ability of the microorganisms present on equipment surfaces both before and after cleaning procedures to contaminate products during processing. Growth of Bacillus biofilms was detectable on both stainless-steel and Teflon© surfaces after all the cleaning procedures. B. cereus and B. subtilis cells adhered more firmly to unsoiled than to soiled surfaces, whereas B. thuringiensis adhered most firmly to soiled surfaces. The results showed that the removal of biofilms from surfaces was more difficult from stainless steel because the microorganisms were attached more tenaciously to rough surfaces. Biofilms were cleaned most effectively from unsoiled surfaces with a simple rinsing procedure; thus the mechanical forces of the flow are very important in the cleaning. The chemical and heat treatments used for cleaning had the greatest impact on the abundance of living microorganisms. All the procedures tested did not remove biofilm material effectively from the surfaces. Significantly more cells were removed when the alkaline phase in the alkaline-acid treatment of the cleaning-inplace (CIP) procedure was prolonged, and ethylene diaminetetraacetic acid (EDTA) was used.

M3 - Article

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SP - 727

EP - 733

JO - Journal of Food Protection

JF - Journal of Food Protection

SN - 0362-028X

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ER -