Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro

M.-P. Gonthier, C. Remesy, A. Scalbert, V. Cheynier, J.-M. Souquet, Kaisa Poutanen, Anna-Marja Aura (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

148 Citations (Scopus)

Abstract

Caffeic acid and its esters, chlorogenic and caftaric acids, are major dietary polyphenols present in various foods and beverages. Although caffeic acid is easily absorbed in the small intestine, its esterification with quinic acid, as in chlorogenic acid, decreases its gut absorption and increases the quantities reaching the colon and its microbiota. The microbial conversion of caftaric acid, the tartaric acid ester of caffeic acid, has not been studied earlier. In this work we compared the direct action of a human faecal microbiota on the metabolism of caffeic, chlorogenic and caftaric acids in an in vitro fermentation model. All substrates disappeared quickly and none of the free acids (caffeic, quinic or tartaric acids) were detected after 2 hours of incubation. Two major microbial metabolites were identified by HPLC–ESI–MS–MS as 3-hydroxyphenylpropionic (3-HPP) and benzoic acids (BA). Maximal levels of 3-HPP were reached after 2 h of fermentation and accounted for 9–24% of the dose of caffeic acid and its esters. BA was formed steadily throughout the incubation, accounting for 4–5% of the initial dose of the substrates after 24 h of incubation. The similarities in the metabolic patterns observed for caffeic, chlorogenic and caftaric acids suggest that esterification does not influence the metabolism of caffeic acid by the gut microbiota.
Original languageEnglish
Pages (from-to)536 - 540
JournalBiomedicine and Pharmacotherapy
Volume60
Issue number9
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Chlorogenic Acid
Microbiota
Esters
Quinic Acid
Esterification
Benzoates
Fermentation
Caffeic Acids
Food and Beverages
Polyphenols
Small Intestine
Colon
In Vitro Techniques
caftaric acid
caffeic acid

Keywords

  • Polyphenols
  • Caffeic acid
  • Chlorogenic acid
  • Caftaric acid
  • Faecal microbiota
  • In vitro metabolism

Cite this

Gonthier, M.-P. ; Remesy, C. ; Scalbert, A. ; Cheynier, V. ; Souquet, J.-M. ; Poutanen, Kaisa ; Aura, Anna-Marja. / Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro. In: Biomedicine and Pharmacotherapy. 2006 ; Vol. 60, No. 9. pp. 536 - 540.
@article{38104a02d15549c6a26a82aa5f5040d9,
title = "Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro",
abstract = "Caffeic acid and its esters, chlorogenic and caftaric acids, are major dietary polyphenols present in various foods and beverages. Although caffeic acid is easily absorbed in the small intestine, its esterification with quinic acid, as in chlorogenic acid, decreases its gut absorption and increases the quantities reaching the colon and its microbiota. The microbial conversion of caftaric acid, the tartaric acid ester of caffeic acid, has not been studied earlier. In this work we compared the direct action of a human faecal microbiota on the metabolism of caffeic, chlorogenic and caftaric acids in an in vitro fermentation model. All substrates disappeared quickly and none of the free acids (caffeic, quinic or tartaric acids) were detected after 2 hours of incubation. Two major microbial metabolites were identified by HPLC–ESI–MS–MS as 3-hydroxyphenylpropionic (3-HPP) and benzoic acids (BA). Maximal levels of 3-HPP were reached after 2 h of fermentation and accounted for 9–24{\%} of the dose of caffeic acid and its esters. BA was formed steadily throughout the incubation, accounting for 4–5{\%} of the initial dose of the substrates after 24 h of incubation. The similarities in the metabolic patterns observed for caffeic, chlorogenic and caftaric acids suggest that esterification does not influence the metabolism of caffeic acid by the gut microbiota.",
keywords = "Polyphenols, Caffeic acid, Chlorogenic acid, Caftaric acid, Faecal microbiota, In vitro metabolism",
author = "M.-P. Gonthier and C. Remesy and A. Scalbert and V. Cheynier and J.-M. Souquet and Kaisa Poutanen and Anna-Marja Aura",
year = "2006",
doi = "10.1016/j.biopha.2006.07.084",
language = "English",
volume = "60",
pages = "536 -- 540",
journal = "Biomedicine and Pharmacotherapy",
issn = "0753-3322",
publisher = "Elsevier",
number = "9",

}

Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro. / Gonthier, M.-P.; Remesy, C.; Scalbert, A.; Cheynier, V.; Souquet, J.-M.; Poutanen, Kaisa; Aura, Anna-Marja (Corresponding Author).

In: Biomedicine and Pharmacotherapy, Vol. 60, No. 9, 2006, p. 536 - 540.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro

AU - Gonthier, M.-P.

AU - Remesy, C.

AU - Scalbert, A.

AU - Cheynier, V.

AU - Souquet, J.-M.

AU - Poutanen, Kaisa

AU - Aura, Anna-Marja

PY - 2006

Y1 - 2006

N2 - Caffeic acid and its esters, chlorogenic and caftaric acids, are major dietary polyphenols present in various foods and beverages. Although caffeic acid is easily absorbed in the small intestine, its esterification with quinic acid, as in chlorogenic acid, decreases its gut absorption and increases the quantities reaching the colon and its microbiota. The microbial conversion of caftaric acid, the tartaric acid ester of caffeic acid, has not been studied earlier. In this work we compared the direct action of a human faecal microbiota on the metabolism of caffeic, chlorogenic and caftaric acids in an in vitro fermentation model. All substrates disappeared quickly and none of the free acids (caffeic, quinic or tartaric acids) were detected after 2 hours of incubation. Two major microbial metabolites were identified by HPLC–ESI–MS–MS as 3-hydroxyphenylpropionic (3-HPP) and benzoic acids (BA). Maximal levels of 3-HPP were reached after 2 h of fermentation and accounted for 9–24% of the dose of caffeic acid and its esters. BA was formed steadily throughout the incubation, accounting for 4–5% of the initial dose of the substrates after 24 h of incubation. The similarities in the metabolic patterns observed for caffeic, chlorogenic and caftaric acids suggest that esterification does not influence the metabolism of caffeic acid by the gut microbiota.

AB - Caffeic acid and its esters, chlorogenic and caftaric acids, are major dietary polyphenols present in various foods and beverages. Although caffeic acid is easily absorbed in the small intestine, its esterification with quinic acid, as in chlorogenic acid, decreases its gut absorption and increases the quantities reaching the colon and its microbiota. The microbial conversion of caftaric acid, the tartaric acid ester of caffeic acid, has not been studied earlier. In this work we compared the direct action of a human faecal microbiota on the metabolism of caffeic, chlorogenic and caftaric acids in an in vitro fermentation model. All substrates disappeared quickly and none of the free acids (caffeic, quinic or tartaric acids) were detected after 2 hours of incubation. Two major microbial metabolites were identified by HPLC–ESI–MS–MS as 3-hydroxyphenylpropionic (3-HPP) and benzoic acids (BA). Maximal levels of 3-HPP were reached after 2 h of fermentation and accounted for 9–24% of the dose of caffeic acid and its esters. BA was formed steadily throughout the incubation, accounting for 4–5% of the initial dose of the substrates after 24 h of incubation. The similarities in the metabolic patterns observed for caffeic, chlorogenic and caftaric acids suggest that esterification does not influence the metabolism of caffeic acid by the gut microbiota.

KW - Polyphenols

KW - Caffeic acid

KW - Chlorogenic acid

KW - Caftaric acid

KW - Faecal microbiota

KW - In vitro metabolism

U2 - 10.1016/j.biopha.2006.07.084

DO - 10.1016/j.biopha.2006.07.084

M3 - Article

VL - 60

SP - 536

EP - 540

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

SN - 0753-3322

IS - 9

ER -