Microbial metabolism of catechin stereoisomers by human faecal microbiota: Comparison of targeted analysis and a non-targeted metabolomics method

Anna-Marja Aura, Ismo Mattila, Tuulikki Seppänen-Laakso, Jarkko Miettinen, Kirsi-Marja Oksman-Caldentey, Matej Oresic

Research output: Contribution to journalArticleScientificpeer-review

59 Citations (Scopus)

Abstract

Microbial metabolism of the stereoisomers (+)-catechin and (−)-epicatechin was compared by two analytical techniques, GC/MS for quantitative targeted analysis and GC×GC-TOF for global characterization of the metabolome, using human faecal microbiota as an inoculum of converting microbiota. The ring-fission site changed when the inocula originated from two different groups of donors, but dehydroxylation progressed similarly regardless of the inoculum. Whereas GC/MS proved to be an appropriate tool for the study of specific expected metabolites of catechin stereoisomers, GC×GC-TOF-based metabolomics analysis also revealed new metabolites not included in the targeted analyses. Quantitation and verification of identification can also be performed in a metabolomics platform, if authentic standards are available.
Original languageEnglish
Pages (from-to)18 - 22
JournalPhytochemistry Letters
Volume1
Issue number1
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Keywords

  • metabolomics
  • colonic microbiota
  • faecal microbiota
  • gastrointestinal (GI) microbiota
  • gut microbiota

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