Microbial metabolism of catechin stereoisomers by human faecal microbiota: Comparison of targeted analysis and a non-targeted metabolomics method

Anna-Marja Aura, Ismo Mattila, Tuulikki Seppänen-Laakso, Jarkko Miettinen, Kirsi-Marja Oksman-Caldentey, Matej Oresic

Research output: Contribution to journalArticleScientificpeer-review

57 Citations (Scopus)

Abstract

Microbial metabolism of the stereoisomers (+)-catechin and (−)-epicatechin was compared by two analytical techniques, GC/MS for quantitative targeted analysis and GC×GC-TOF for global characterization of the metabolome, using human faecal microbiota as an inoculum of converting microbiota. The ring-fission site changed when the inocula originated from two different groups of donors, but dehydroxylation progressed similarly regardless of the inoculum. Whereas GC/MS proved to be an appropriate tool for the study of specific expected metabolites of catechin stereoisomers, GC×GC-TOF-based metabolomics analysis also revealed new metabolites not included in the targeted analyses. Quantitation and verification of identification can also be performed in a metabolomics platform, if authentic standards are available.
Original languageEnglish
Pages (from-to)18 - 22
JournalPhytochemistry Letters
Volume1
Issue number1
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

stereoisomers
Stereoisomerism
Metabolomics
Catechin
Microbiota
metabolomics
catechin
Metabolism
inoculum
Metabolites
metabolism
metabolites
metabolome
Metabolome
epicatechin
analytical methods
methodology
microbiome

Keywords

  • metabolomics
  • colonic microbiota
  • faecal microbiota
  • gastrointestinal (GI) microbiota
  • gut microbiota

Cite this

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title = "Microbial metabolism of catechin stereoisomers by human faecal microbiota: Comparison of targeted analysis and a non-targeted metabolomics method",
abstract = "Microbial metabolism of the stereoisomers (+)-catechin and (−)-epicatechin was compared by two analytical techniques, GC/MS for quantitative targeted analysis and GC×GC-TOF for global characterization of the metabolome, using human faecal microbiota as an inoculum of converting microbiota. The ring-fission site changed when the inocula originated from two different groups of donors, but dehydroxylation progressed similarly regardless of the inoculum. Whereas GC/MS proved to be an appropriate tool for the study of specific expected metabolites of catechin stereoisomers, GC×GC-TOF-based metabolomics analysis also revealed new metabolites not included in the targeted analyses. Quantitation and verification of identification can also be performed in a metabolomics platform, if authentic standards are available.",
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Microbial metabolism of catechin stereoisomers by human faecal microbiota: Comparison of targeted analysis and a non-targeted metabolomics method. / Aura, Anna-Marja; Mattila, Ismo; Seppänen-Laakso, Tuulikki; Miettinen, Jarkko; Oksman-Caldentey, Kirsi-Marja; Oresic, Matej.

In: Phytochemistry Letters, Vol. 1, No. 1, 2008, p. 18 - 22.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Microbial metabolism of catechin stereoisomers by human faecal microbiota: Comparison of targeted analysis and a non-targeted metabolomics method

AU - Aura, Anna-Marja

AU - Mattila, Ismo

AU - Seppänen-Laakso, Tuulikki

AU - Miettinen, Jarkko

AU - Oksman-Caldentey, Kirsi-Marja

AU - Oresic, Matej

PY - 2008

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AB - Microbial metabolism of the stereoisomers (+)-catechin and (−)-epicatechin was compared by two analytical techniques, GC/MS for quantitative targeted analysis and GC×GC-TOF for global characterization of the metabolome, using human faecal microbiota as an inoculum of converting microbiota. The ring-fission site changed when the inocula originated from two different groups of donors, but dehydroxylation progressed similarly regardless of the inoculum. Whereas GC/MS proved to be an appropriate tool for the study of specific expected metabolites of catechin stereoisomers, GC×GC-TOF-based metabolomics analysis also revealed new metabolites not included in the targeted analyses. Quantitation and verification of identification can also be performed in a metabolomics platform, if authentic standards are available.

KW - metabolomics

KW - colonic microbiota

KW - faecal microbiota

KW - gastrointestinal (GI) microbiota

KW - gut microbiota

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