Abstract
Cleaning procedures with or without disinfectant
treatment for both open and closed systems were used in
developing an assessment system based on various
microbial methods for detection of residual
surface-attached microbes and soil on surfaces after
sanitation. The microbial methods tested were
cultivation, microscopy using image analysis after
staining with acridine orange, viability staining,
impedance measurements, viable counts with TTC agar and
ATP measurements. Conventional cultivation, combined with
impedance measurements and image analysis of surfaces
stained with acridine orange gave results that were
comparable and complementary. Combination of these
methods enabled a total evaluation of both the removal of
biofilm and the killing of bacterial cells. Some of the
cleaning procedures tested neither removed all of the
bacterial cells from the surfaces nor killed the
bacteria. The above-mentioned testing protocol can also
be used for evaluating the sensitivity of microbial
testing methods for use in certain industrial premises.
| Original language | English |
|---|---|
| Title of host publication | 30th R3-Nordic Contamination Control Symposium |
| Place of Publication | Espoo |
| Publisher | VTT Technical Research Centre of Finland |
| Pages | 207-218 |
| ISBN (Print) | 951-38-5268-7 |
| Publication status | Published - 1999 |
| MoE publication type | A4 Article in a conference publication |
| Event | 30th R3-Nordic Contamination Control Symposium - Helsinki, Finland Duration: 30 May 1999 → 2 Jun 1999 |
Conference
| Conference | 30th R3-Nordic Contamination Control Symposium |
|---|---|
| Country/Territory | Finland |
| City | Helsinki |
| Period | 30/05/99 → 2/06/99 |