TY - JOUR
T1 - Microbial populations on brewery filling hall surfaces: Progress towards functional coatings
AU - Priha, Outi
AU - Raulio, Mari
AU - Cooke, Kevin
AU - Fisher, Leanne
AU - Hill, Claire
AU - Hylkinen, Silja
AU - Kelly, Peter
AU - Navabpour, Parnia
AU - Ostovarpour, Soheyla
AU - Tapani, Kaisa
AU - Tattershall, Carin
AU - Vehviläinen, Anna-Kaisa
AU - Verran, Joanna
AU - Storgårds, Erna
PY - 2015
Y1 - 2015
N2 - Microbial populations on equipment surfaces of beverage
filling lines were investigated as a function of surface
coating type, location and time. Photocatalytic metal-ion
doped (Ag or Mo) and non-doped TiO2 coatings deposited
using reactive magnetron sputtering and spray coating
methods were studied as means to reduce microbial numbers
accumulating on the surfaces. The coatings were applied
to stainless steel coupons, which were mounted to one
canning and one glass bottle filling line for 3-5 months.
After exposure microbial numbers on the coupons were
evaluated by culturing, and bacterial community profiles
were characterised with PCR-DGGE (denaturing gradient gel
electrophoresis). The results showed that the longer the
run time after washes the higher microbial numbers were
detected, and that the two filling lines each had their
characteristic bacterial community. The major species
identified were members of Acinetobacter sp., lactic acid
bacteria and enterobacteria. No clear effect of the
different coating materials on the microbial numbers or
bacterial community composition on the surfaces was
shown. In conclusion, functional coatings with sufficient
mechanical and chemical durability for industrial
surfaces have been developed. Although these coatings
have been previously reported to reduce the number of
microorganisms on the surfaces in vitro, their efficacy
in the challenging beverage process conditions was not
proven.
AB - Microbial populations on equipment surfaces of beverage
filling lines were investigated as a function of surface
coating type, location and time. Photocatalytic metal-ion
doped (Ag or Mo) and non-doped TiO2 coatings deposited
using reactive magnetron sputtering and spray coating
methods were studied as means to reduce microbial numbers
accumulating on the surfaces. The coatings were applied
to stainless steel coupons, which were mounted to one
canning and one glass bottle filling line for 3-5 months.
After exposure microbial numbers on the coupons were
evaluated by culturing, and bacterial community profiles
were characterised with PCR-DGGE (denaturing gradient gel
electrophoresis). The results showed that the longer the
run time after washes the higher microbial numbers were
detected, and that the two filling lines each had their
characteristic bacterial community. The major species
identified were members of Acinetobacter sp., lactic acid
bacteria and enterobacteria. No clear effect of the
different coating materials on the microbial numbers or
bacterial community composition on the surfaces was
shown. In conclusion, functional coatings with sufficient
mechanical and chemical durability for industrial
surfaces have been developed. Although these coatings
have been previously reported to reduce the number of
microorganisms on the surfaces in vitro, their efficacy
in the challenging beverage process conditions was not
proven.
KW - beverage industry
KW - microbial communities
KW - photocatalytic coatings
KW - bacteria
KW - process surfaces
U2 - 10.1016/j.foodcont.2015.02.022
DO - 10.1016/j.foodcont.2015.02.022
M3 - Article
SN - 0956-7135
VL - 55
SP - 1
EP - 11
JO - Food Control
JF - Food Control
ER -