Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland

Hanna Miettinen; Mari Nyyssönen; Maija Nuppunen-Puputti; Riikka Kietäväinen; Ilmo Kukkonen; Ville Nenonen; Aino Karjalainen; Jussi Rytkönen; Malin Bomberg

Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland

Hanna Miettinen^*, Mari Nyyssönen, Maija Nuppunen-Puputti, Riikka Kietäväinen, Ilmo Kukkonen, Ville Nenonen, Aino Karjalainen, Jussi Rytkönen, Malin Bomberg

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

Abstract

Espoo superdeep boreholes OTN2 and OTN3 were sampled in multidisciplinary collaboration. Microbiological part focused on obtaining microbiologically reliable uncontaminated fluid samples from the depth of 5 km where microbial communities were expected to be sparse. Several aspects needed to be considered in securing viable microorganisms such as high pressure and salinity as well as keeping the samples anoxic. Contamination control was essential including both extensive disinfection of sampling devices but also negative controls. The main objectives of microbial sampling were to detect and identify microbial communities present in the superdeep subsurface with DNA-based methods and to grow and identify possible viable microbial cells.

Original language	English
Title of host publication	Lithospere 2024
Subtitle of host publication	Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere
Editors	Perttu Mikkola, Toni Veikkolainen, Suvi Heinonen, Hannu Koivula, Kaisa Nikkilä, Jochen Kamm, David Whipp, Riikka Kietäväinen, Hanna Silvennoinen
Publisher	University of Helsinki
Pages	64-67
ISBN (Electronic)	978-952-10-9614-3
Publication status	Published - 2024
MoE publication type	B3 Non-refereed article in conference proceedings
Event	13th Symposium on Structure, Composition and Evolution of the Lithosphere in Fennoscandia, LITO 2024 - GTK, Otaniemi, Espoo, Finland Duration: 12 Nov 2024 → 14 Nov 2024 https://www.seismo.helsinki.fi/ilp/lito2024/

Publication series

Series	University of Helsinki: Institute of Seismology report
Number	S-73
ISSN	0357-3060

Conference

Conference	13th Symposium on Structure, Composition and Evolution of the Lithosphere in Fennoscandia, LITO 2024
Country/Territory	Finland
City	Espoo
Period	12/11/24 → 14/11/24
Internet address	https://www.seismo.helsinki.fi/ilp/lito2024/

Funding

DAFNE-22: Deep life, hydrogeology and geothermics in the ICDP-DAFNE scientific drilling project

Keywords

high temperature
high pressure
bacteria
archaea
salinity
biosphere
subsurface

Access to Document

https://www.seismo.helsinki.fi/pdf/Lito2024.pdf#page=79

Cite this

Miettinen, H., Nyyssönen, M., Nuppunen-Puputti, M., Kietäväinen, R., Kukkonen, I., Nenonen, V., Karjalainen, A., Rytkönen, J., & Bomberg, M. (2024). Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland. In P. Mikkola, T. Veikkolainen, S. Heinonen, H. Koivula, K. Nikkilä, J. Kamm, D. Whipp, R. Kietäväinen, & H. Silvennoinen (Eds.), Lithospere 2024: Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere (pp. 64-67). University of Helsinki. https://www.seismo.helsinki.fi/pdf/Lito2024.pdf#page=79

Miettinen, Hanna ; Nyyssönen, Mari ; Nuppunen-Puputti, Maija et al. / Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland. Lithospere 2024: Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere. editor / Perttu Mikkola ; Toni Veikkolainen ; Suvi Heinonen ; Hannu Koivula ; Kaisa Nikkilä ; Jochen Kamm ; David Whipp ; Riikka Kietäväinen ; Hanna Silvennoinen. University of Helsinki, 2024. pp. 64-67 (University of Helsinki: Institute of Seismology report; No. S-73).

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title = "Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland",

abstract = "Espoo superdeep boreholes OTN2 and OTN3 were sampled in multidisciplinary collaboration. Microbiological part focused on obtaining microbiologically reliable uncontaminated fluid samples from the depth of 5 km where microbial communities were expected to be sparse. Several aspects needed to be considered in securing viable microorganisms such as high pressure and salinity as well as keeping the samples anoxic. Contamination control was essential including both extensive disinfection of sampling devices but also negative controls. The main objectives of microbial sampling were to detect and identify microbial communities present in the superdeep subsurface with DNA-based methods and to grow and identify possible viable microbial cells.",

keywords = "high temperature, high pressure, bacteria, archaea, salinity, biosphere, subsurface",

author = "Hanna Miettinen and Mari Nyyss{\"o}nen and Maija Nuppunen-Puputti and Riikka Kiet{\"a}v{\"a}inen and Ilmo Kukkonen and Ville Nenonen and Aino Karjalainen and Jussi Rytk{\"o}nen and Malin Bomberg",

note = "extended abstract; 13th Symposium on Structure, Composition and Evolution of the Lithosphere in Fennoscandia, LITO 2024 ; Conference date: 12-11-2024 Through 14-11-2024",

year = "2024",

language = "English",

series = "University of Helsinki: Institute of Seismology report",

publisher = "University of Helsinki",

number = "S-73",

pages = "64--67",

editor = "Perttu Mikkola and Toni Veikkolainen and Suvi Heinonen and Hannu Koivula and Kaisa Nikkil{\"a} and Jochen Kamm and David Whipp and Riikka Kiet{\"a}v{\"a}inen and Hanna Silvennoinen",

booktitle = "Lithospere 2024",

address = "Finland",

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Miettinen, H , Nyyssönen, M , Nuppunen-Puputti, M, Kietäväinen, R, Kukkonen, I, Nenonen, V, Karjalainen, A, Rytkönen, J & Bomberg, M 2024, Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland. in P Mikkola, T Veikkolainen, S Heinonen, H Koivula, K Nikkilä, J Kamm, D Whipp, R Kietäväinen & H Silvennoinen (eds), Lithospere 2024: Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere. University of Helsinki, University of Helsinki: Institute of Seismology report, no. S-73, pp. 64-67, 13th Symposium on Structure, Composition and Evolution of the Lithosphere in Fennoscandia, LITO 2024, Espoo, Finland, 12/11/24. <https://www.seismo.helsinki.fi/pdf/Lito2024.pdf#page=79>

Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland. / Miettinen, Hanna ; Nyyssönen, Mari ; Nuppunen-Puputti, Maija et al.
Lithospere 2024: Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere. ed. / Perttu Mikkola; Toni Veikkolainen; Suvi Heinonen; Hannu Koivula; Kaisa Nikkilä; Jochen Kamm; David Whipp; Riikka Kietäväinen; Hanna Silvennoinen. University of Helsinki, 2024. p. 64-67 (University of Helsinki: Institute of Seismology report; No. S-73).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

TY - GEN

T1 - Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland

AU - Miettinen, Hanna

AU - Nyyssönen, Mari

AU - Nuppunen-Puputti, Maija

AU - Kietäväinen, Riikka

AU - Kukkonen, Ilmo

AU - Nenonen, Ville

AU - Karjalainen, Aino

AU - Rytkönen, Jussi

AU - Bomberg, Malin

N1 - extended abstract

PY - 2024

Y1 - 2024

N2 - Espoo superdeep boreholes OTN2 and OTN3 were sampled in multidisciplinary collaboration. Microbiological part focused on obtaining microbiologically reliable uncontaminated fluid samples from the depth of 5 km where microbial communities were expected to be sparse. Several aspects needed to be considered in securing viable microorganisms such as high pressure and salinity as well as keeping the samples anoxic. Contamination control was essential including both extensive disinfection of sampling devices but also negative controls. The main objectives of microbial sampling were to detect and identify microbial communities present in the superdeep subsurface with DNA-based methods and to grow and identify possible viable microbial cells.

AB - Espoo superdeep boreholes OTN2 and OTN3 were sampled in multidisciplinary collaboration. Microbiological part focused on obtaining microbiologically reliable uncontaminated fluid samples from the depth of 5 km where microbial communities were expected to be sparse. Several aspects needed to be considered in securing viable microorganisms such as high pressure and salinity as well as keeping the samples anoxic. Contamination control was essential including both extensive disinfection of sampling devices but also negative controls. The main objectives of microbial sampling were to detect and identify microbial communities present in the superdeep subsurface with DNA-based methods and to grow and identify possible viable microbial cells.

KW - high temperature

KW - high pressure

KW - bacteria

KW - archaea

KW - salinity

KW - biosphere

KW - subsurface

M3 - Conference article in proceedings

T3 - University of Helsinki: Institute of Seismology report

SP - 64

EP - 67

BT - Lithospere 2024

A2 - Mikkola, Perttu

A2 - Veikkolainen, Toni

A2 - Heinonen, Suvi

A2 - Koivula, Hannu

A2 - Nikkilä, Kaisa

A2 - Kamm, Jochen

A2 - Whipp, David

A2 - Kietäväinen, Riikka

A2 - Silvennoinen, Hanna

PB - University of Helsinki

T2 - 13th Symposium on Structure, Composition and Evolution of the Lithosphere in Fennoscandia, LITO 2024

Y2 - 12 November 2024 through 14 November 2024

ER -

Miettinen H , Nyyssönen M , Nuppunen-Puputti M, Kietäväinen R, Kukkonen I, Nenonen V et al. Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland. In Mikkola P, Veikkolainen T, Heinonen S, Koivula H, Nikkilä K, Kamm J, Whipp D, Kietäväinen R, Silvennoinen H, editors, Lithospere 2024: Thirteenth Symposium on the Structure, Composition and Evolution of the Lithosphere. University of Helsinki. 2024. p. 64-67. (University of Helsinki: Institute of Seismology report; No. S-73).

Microbiological aspects of sampling the St1 superdeep boreholes in Espoo, southern Finland

Abstract

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Conference

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Keywords

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