Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008

Merja Itävaara, Mari Nyyssönen, Malin Bomberg, Anu Kapanen, Aura Nousiainen, Lasse Ahonen, Jenni Hultman, Lars Paulin, Petri Auvinen, Ilmo T. Kukkonen

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

Three sampling campaigns were performed during the years 2007-2009 to develop microbiological sampling techniques and to study the deep biosphere in the Outokumpu Deep Drill Hole R2500, drilled into a Palaeoproterozoic sequence of rocks consisting of metasediments, ophiolite-derived altered ultramafic rocks and pegmatitic granite. The results revealed the presence of microbial communities in the saline fluids sampled from the open borehole and fracture systems in the low-porosity crystalline bedrock in Outokumpu. According to the microbiological analyses, the microbial cell density varies from 105 cells ml-1 at the surface to 103 ml-1 at 2350 m depth. The composition of microbial communities in the borehole was found to vary with sampling depth, and the changes appear to be connected to both geological and geochemical factors as well as to fracture zones in the bedrock. Sulphate reducing bacteria and methanogenic archaea are present throughout the borehole water column.
Original languageEnglish
Title of host publicationOutokumpu Deep Drilling Project 2003-2010
EditorsIlmo T. Kukkonen
Place of PublicationEspoo
PublisherGeological Survey of Finland
Pages199-206
ISBN (Electronic)978-952-217-152-8
ISBN (Print)978-952-217-151-1
Publication statusPublished - 2011
MoE publication typeA3 Part of a book or another research book

Publication series

SeriesGeological Survey of Finland. Special Paper
Number51
ISSN0782-8535

Fingerprint

biosphere
borehole
microbial community
bedrock
sampling
sulfate-reducing bacterium
metasediment
ultramafic rock
fracture zone
ophiolite
granite
water column
porosity
fluid
rock
analysis
sampling technique

Keywords

  • deep drilling
  • bedrock
  • boreholes
  • ground water
  • biosphere
  • microorganisms
  • bacteria
  • Archaea
  • Outokumpu
  • Finland

Cite this

Itävaara, M., Nyyssönen, M., Bomberg, M., Kapanen, A., Nousiainen, A., Ahonen, L., ... Kukkonen, I. T. (2011). Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008. In I. T. Kukkonen (Ed.), Outokumpu Deep Drilling Project 2003-2010 (pp. 199-206). Espoo: Geological Survey of Finland. Geological Survey of Finland. Special Paper, No. 51
Itävaara, Merja ; Nyyssönen, Mari ; Bomberg, Malin ; Kapanen, Anu ; Nousiainen, Aura ; Ahonen, Lasse ; Hultman, Jenni ; Paulin, Lars ; Auvinen, Petri ; Kukkonen, Ilmo T. / Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008. Outokumpu Deep Drilling Project 2003-2010. editor / Ilmo T. Kukkonen. Espoo : Geological Survey of Finland, 2011. pp. 199-206 (Geological Survey of Finland. Special Paper; No. 51).
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abstract = "Three sampling campaigns were performed during the years 2007-2009 to develop microbiological sampling techniques and to study the deep biosphere in the Outokumpu Deep Drill Hole R2500, drilled into a Palaeoproterozoic sequence of rocks consisting of metasediments, ophiolite-derived altered ultramafic rocks and pegmatitic granite. The results revealed the presence of microbial communities in the saline fluids sampled from the open borehole and fracture systems in the low-porosity crystalline bedrock in Outokumpu. According to the microbiological analyses, the microbial cell density varies from 105 cells ml-1 at the surface to 103 ml-1 at 2350 m depth. The composition of microbial communities in the borehole was found to vary with sampling depth, and the changes appear to be connected to both geological and geochemical factors as well as to fracture zones in the bedrock. Sulphate reducing bacteria and methanogenic archaea are present throughout the borehole water column.",
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author = "Merja It{\"a}vaara and Mari Nyyss{\"o}nen and Malin Bomberg and Anu Kapanen and Aura Nousiainen and Lasse Ahonen and Jenni Hultman and Lars Paulin and Petri Auvinen and Kukkonen, {Ilmo T.}",
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Itävaara, M, Nyyssönen, M, Bomberg, M, Kapanen, A, Nousiainen, A, Ahonen, L, Hultman, J, Paulin, L, Auvinen, P & Kukkonen, IT 2011, Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008. in IT Kukkonen (ed.), Outokumpu Deep Drilling Project 2003-2010. Geological Survey of Finland, Espoo, Geological Survey of Finland. Special Paper, no. 51, pp. 199-206.

Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008. / Itävaara, Merja; Nyyssönen, Mari; Bomberg, Malin; Kapanen, Anu; Nousiainen, Aura; Ahonen, Lasse; Hultman, Jenni; Paulin, Lars; Auvinen, Petri; Kukkonen, Ilmo T.

Outokumpu Deep Drilling Project 2003-2010. ed. / Ilmo T. Kukkonen. Espoo : Geological Survey of Finland, 2011. p. 199-206 (Geological Survey of Finland. Special Paper; No. 51).

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

TY - CHAP

T1 - Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008

AU - Itävaara, Merja

AU - Nyyssönen, Mari

AU - Bomberg, Malin

AU - Kapanen, Anu

AU - Nousiainen, Aura

AU - Ahonen, Lasse

AU - Hultman, Jenni

AU - Paulin, Lars

AU - Auvinen, Petri

AU - Kukkonen, Ilmo T.

PY - 2011

Y1 - 2011

N2 - Three sampling campaigns were performed during the years 2007-2009 to develop microbiological sampling techniques and to study the deep biosphere in the Outokumpu Deep Drill Hole R2500, drilled into a Palaeoproterozoic sequence of rocks consisting of metasediments, ophiolite-derived altered ultramafic rocks and pegmatitic granite. The results revealed the presence of microbial communities in the saline fluids sampled from the open borehole and fracture systems in the low-porosity crystalline bedrock in Outokumpu. According to the microbiological analyses, the microbial cell density varies from 105 cells ml-1 at the surface to 103 ml-1 at 2350 m depth. The composition of microbial communities in the borehole was found to vary with sampling depth, and the changes appear to be connected to both geological and geochemical factors as well as to fracture zones in the bedrock. Sulphate reducing bacteria and methanogenic archaea are present throughout the borehole water column.

AB - Three sampling campaigns were performed during the years 2007-2009 to develop microbiological sampling techniques and to study the deep biosphere in the Outokumpu Deep Drill Hole R2500, drilled into a Palaeoproterozoic sequence of rocks consisting of metasediments, ophiolite-derived altered ultramafic rocks and pegmatitic granite. The results revealed the presence of microbial communities in the saline fluids sampled from the open borehole and fracture systems in the low-porosity crystalline bedrock in Outokumpu. According to the microbiological analyses, the microbial cell density varies from 105 cells ml-1 at the surface to 103 ml-1 at 2350 m depth. The composition of microbial communities in the borehole was found to vary with sampling depth, and the changes appear to be connected to both geological and geochemical factors as well as to fracture zones in the bedrock. Sulphate reducing bacteria and methanogenic archaea are present throughout the borehole water column.

KW - deep drilling

KW - bedrock

KW - boreholes

KW - ground water

KW - biosphere

KW - microorganisms

KW - bacteria

KW - Archaea

KW - Outokumpu

KW - Finland

M3 - Chapter or book article

SN - 978-952-217-151-1

T3 - Geological Survey of Finland. Special Paper

SP - 199

EP - 206

BT - Outokumpu Deep Drilling Project 2003-2010

A2 - Kukkonen, Ilmo T.

PB - Geological Survey of Finland

CY - Espoo

ER -

Itävaara M, Nyyssönen M, Bomberg M, Kapanen A, Nousiainen A, Ahonen L et al. Microbiological sampling and analysis of the Outokumpu Deep Borehole biosphere in 2007-2008. In Kukkonen IT, editor, Outokumpu Deep Drilling Project 2003-2010. Espoo: Geological Survey of Finland. 2011. p. 199-206. (Geological Survey of Finland. Special Paper; No. 51).