Microemulsion electrokinetic chromatographic analysis of some polar compounds

Heli Sirén (Corresponding Author), Anne Karttunen

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)


This study presents the optimization of a microemulsion electrokinetic chromatographic (MEEKC) electrolyte solution by using UV detection and with the method, simultaneous separations of chemically, biochemically and pharmaceutically related anionic and cationic compounds. Representatives of the compound groups were from isoflavonoids, benzodiazepines, metanephrines, diuretics and peptide hormones. The MEEKC separations under basic conditions were first optimized using a two-component isoflavonoid mixture as the sample and an electrolyte containing 10 mM tetraborate as the main buffer (pH 9.5). The stable microemulsion phase was adjusted with various amounts of octane, 1-butanol and sodium dodecyl sulfate (SDS). An only acidified electrolyte solution used in the study was made of phosphoric acid (pH 1.8) containing octane, SDS and ethyl acetate. The analyses with isoflavonoids showed that electrophoretic mobilities of the investigated compounds were highly related to the concentrations of SDS and 1-butanol with linear and parabolic correlation, respectively. However, addition of octane gave linear correlation only at low concentrations. In most cases four to six structurally related compounds and even 13 diuretics with various polar properties were separated from each other in basic microemulsion medium. The acidified MEEKC electrolyte gave good resolution for anionic metanephrines.
Original languageEnglish
Pages (from-to)113-124
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number1
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed


  • microemulsionelectrokinetic capillary chromatography
  • drugs


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