Abstract
We have developed a microfluidics based sampling system
for tissue analytics. The proof-of-concept of the
sampling system was demonstrated by extracting lipid
samples from tissue biopsies. The sample collection
system consists of a disposable silicon based multiport
microneedle integrated with polymer microfluidics. The
polymethyl methacrylate polymer microfluidic chip has a
10 ll sample reservoir and actuation membranes for liquid
pumping. A special automated robotic system was developed
to control the positioning of the needle and the sampling
procedure on preselected spots on the tissue. Real breast
cancer tissue samples were used to test the feasibility
of the sampling system. We successfully measured
indicative cancer biomarkers from the tissue surface.
Phosphatidylcholine and phosphoethanolamine were
extracted from the tissue membrane with methyl tert-butyl
ether solvent and detected by mass spectrometry. In the
future, this tool could be used in characterization of
preoperative biopsies and tumour tissues removed during
surgery.
| Original language | English |
|---|---|
| Number of pages | 12 |
| Journal | Biomicrofluidics |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2015 |
| MoE publication type | A1 Journal article-refereed |
