Microfluidic sampling system for tissue analytics

Ari Hokkanen, Ingmar Stuns, Philipp Schmidt, Annukka Kokkonen, Feng Gao, Alexander Steinecker, Jan Budczies, Päivi Heimala, Leena Hakalahti

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

We have developed a microfluidics based sampling system for tissue analytics. The proof-of-concept of the sampling system was demonstrated by extracting lipid samples from tissue biopsies. The sample collection system consists of a disposable silicon based multiport microneedle integrated with polymer microfluidics. The polymethyl methacrylate polymer microfluidic chip has a 10 ll sample reservoir and actuation membranes for liquid pumping. A special automated robotic system was developed to control the positioning of the needle and the sampling procedure on preselected spots on the tissue. Real breast cancer tissue samples were used to test the feasibility of the sampling system. We successfully measured indicative cancer biomarkers from the tissue surface. Phosphatidylcholine and phosphoethanolamine were extracted from the tissue membrane with methyl tert-butyl ether solvent and detected by mass spectrometry. In the future, this tool could be used in characterization of preoperative biopsies and tumour tissues removed during surgery.
Original languageEnglish
Number of pages12
JournalBiomicrofluidics
Volume9
Issue number5
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Microfluidics
sampling
Tissue
Sampling
Biopsy
Polymers
cancer
Membranes
membranes
Robotics
Polymethyl Methacrylate
Silicon
Tumor Biomarkers
Phosphatidylcholines
biomarkers
polymers
Needles
robotics
Polymethyl methacrylates
actuation

Cite this

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title = "Microfluidic sampling system for tissue analytics",
abstract = "We have developed a microfluidics based sampling system for tissue analytics. The proof-of-concept of the sampling system was demonstrated by extracting lipid samples from tissue biopsies. The sample collection system consists of a disposable silicon based multiport microneedle integrated with polymer microfluidics. The polymethyl methacrylate polymer microfluidic chip has a 10 ll sample reservoir and actuation membranes for liquid pumping. A special automated robotic system was developed to control the positioning of the needle and the sampling procedure on preselected spots on the tissue. Real breast cancer tissue samples were used to test the feasibility of the sampling system. We successfully measured indicative cancer biomarkers from the tissue surface. Phosphatidylcholine and phosphoethanolamine were extracted from the tissue membrane with methyl tert-butyl ether solvent and detected by mass spectrometry. In the future, this tool could be used in characterization of preoperative biopsies and tumour tissues removed during surgery.",
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Microfluidic sampling system for tissue analytics. / Hokkanen, Ari; Stuns, Ingmar; Schmidt, Philipp; Kokkonen, Annukka; Gao, Feng; Steinecker, Alexander; Budczies, Jan; Heimala, Päivi; Hakalahti, Leena.

In: Biomicrofluidics, Vol. 9, No. 5, 2015.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Microfluidic sampling system for tissue analytics

AU - Hokkanen, Ari

AU - Stuns, Ingmar

AU - Schmidt, Philipp

AU - Kokkonen, Annukka

AU - Gao, Feng

AU - Steinecker, Alexander

AU - Budczies, Jan

AU - Heimala, Päivi

AU - Hakalahti, Leena

N1 - Project code: 40879

PY - 2015

Y1 - 2015

N2 - We have developed a microfluidics based sampling system for tissue analytics. The proof-of-concept of the sampling system was demonstrated by extracting lipid samples from tissue biopsies. The sample collection system consists of a disposable silicon based multiport microneedle integrated with polymer microfluidics. The polymethyl methacrylate polymer microfluidic chip has a 10 ll sample reservoir and actuation membranes for liquid pumping. A special automated robotic system was developed to control the positioning of the needle and the sampling procedure on preselected spots on the tissue. Real breast cancer tissue samples were used to test the feasibility of the sampling system. We successfully measured indicative cancer biomarkers from the tissue surface. Phosphatidylcholine and phosphoethanolamine were extracted from the tissue membrane with methyl tert-butyl ether solvent and detected by mass spectrometry. In the future, this tool could be used in characterization of preoperative biopsies and tumour tissues removed during surgery.

AB - We have developed a microfluidics based sampling system for tissue analytics. The proof-of-concept of the sampling system was demonstrated by extracting lipid samples from tissue biopsies. The sample collection system consists of a disposable silicon based multiport microneedle integrated with polymer microfluidics. The polymethyl methacrylate polymer microfluidic chip has a 10 ll sample reservoir and actuation membranes for liquid pumping. A special automated robotic system was developed to control the positioning of the needle and the sampling procedure on preselected spots on the tissue. Real breast cancer tissue samples were used to test the feasibility of the sampling system. We successfully measured indicative cancer biomarkers from the tissue surface. Phosphatidylcholine and phosphoethanolamine were extracted from the tissue membrane with methyl tert-butyl ether solvent and detected by mass spectrometry. In the future, this tool could be used in characterization of preoperative biopsies and tumour tissues removed during surgery.

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