Microsystems for Biological Cell Characterization: Dissertation

Anna Rissanen

    Research output: ThesisDissertationMonograph

    Abstract

    This thesis describes three techniques for the characterization of living cells using micro-electro-mechanical systems (MEMS) based devices. The study of cellular function and structure is essential for bioprocess control, disease diagnosis, patient treatment and drug discovery. Microsystem technology enables characterization of very small samples, minimal use of expensive reagents, testing of multiple samples in parallel, and point-of-care testing, all of which increase throughput and reduce the analysis cost. The three characterization techniques presented in this thesis could be integrated into a microfluidic cellular total analysis system to obtain complementary information of cellular function. The first part of the thesis presents the characterization of bovine adrenal cortex capillary endothelial cells by impedance spectroscopy in a microsystem which was realized using microfabrication techniques. The microsystem consists of a small-volume cell culture area defined on PDMS walls on a glass substrate with gold electrodes coated with a self-assembled monolayer to enable cell attachment. As the main result, it was possible to monitor the capillary formation of BACC endothelial cells in a microsystem using impedance spectroscopy. The second part describes calorimetric characterization of Saccharomyces cerevisiae yeast cells using a MEMS-based nanocalorimetric microsensor. The cells are introduced to the sensor membrane in small droplets (~1 µl), and the sensor thermopile voltage output is compared to the output of the reference water droplet to extract the effect of sample evaporation. The third part describes the design, process integration and fabrication of an electrically tunable Fabry-Perot interferometer (FPI) monolithically integrated on a photodiode for visible spectrum measurements. The options for the process integration of separate FPI optical filters are presented. The application of miniature spectrometers based on MEMS FPI technology in biological cell characterization is discussed.
    Original languageEnglish
    QualificationDoctor Degree
    Awarding Institution
    • Aalto University
    Supervisors/Advisors
    • Tittonen, Ilkka, Supervisor, External person
    • Franssila, Sami, Advisor, External person
    Award date19 Oct 2012
    Place of PublicationEspoo
    Publisher
    Print ISBNs978-951-38-7475-9
    Electronic ISBNs978-951-38-7931-0
    Publication statusPublished - 2012
    MoE publication typeG4 Doctoral dissertation (monograph)

    Fingerprint

    Microsystems
    Fabry-Perot interferometers
    Endothelial cells
    Yeast
    Cells
    Spectroscopy
    Patient treatment
    Thermopiles
    Disease control
    Microsensors
    Optical filters
    Microfabrication
    Sensors
    Testing
    Self assembled monolayers
    Photodiodes
    Cell culture
    Microfluidics
    Gold
    Spectrometers

    Keywords

    • BioMEMS
    • impedance spectroscopy
    • MEMS nanocalorimeter
    • Fabry-Perot interferometer
    • microspectrometers
    • Saccharomyces cerevisiae
    • yeast
    • cell measurement

    Cite this

    Rissanen, A. (2012). Microsystems for Biological Cell Characterization: Dissertation. Espoo: VTT Technical Research Centre of Finland.
    Rissanen, Anna. / Microsystems for Biological Cell Characterization : Dissertation. Espoo : VTT Technical Research Centre of Finland, 2012. 136 p.
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    abstract = "This thesis describes three techniques for the characterization of living cells using micro-electro-mechanical systems (MEMS) based devices. The study of cellular function and structure is essential for bioprocess control, disease diagnosis, patient treatment and drug discovery. Microsystem technology enables characterization of very small samples, minimal use of expensive reagents, testing of multiple samples in parallel, and point-of-care testing, all of which increase throughput and reduce the analysis cost. The three characterization techniques presented in this thesis could be integrated into a microfluidic cellular total analysis system to obtain complementary information of cellular function. The first part of the thesis presents the characterization of bovine adrenal cortex capillary endothelial cells by impedance spectroscopy in a microsystem which was realized using microfabrication techniques. The microsystem consists of a small-volume cell culture area defined on PDMS walls on a glass substrate with gold electrodes coated with a self-assembled monolayer to enable cell attachment. As the main result, it was possible to monitor the capillary formation of BACC endothelial cells in a microsystem using impedance spectroscopy. The second part describes calorimetric characterization of Saccharomyces cerevisiae yeast cells using a MEMS-based nanocalorimetric microsensor. The cells are introduced to the sensor membrane in small droplets (~1 µl), and the sensor thermopile voltage output is compared to the output of the reference water droplet to extract the effect of sample evaporation. The third part describes the design, process integration and fabrication of an electrically tunable Fabry-Perot interferometer (FPI) monolithically integrated on a photodiode for visible spectrum measurements. The options for the process integration of separate FPI optical filters are presented. The application of miniature spectrometers based on MEMS FPI technology in biological cell characterization is discussed.",
    keywords = "BioMEMS, impedance spectroscopy, MEMS nanocalorimeter, Fabry-Perot interferometer, microspectrometers, Saccharomyces cerevisiae, yeast, cell measurement",
    author = "Anna Rissanen",
    note = "Project code: 78365",
    year = "2012",
    language = "English",
    isbn = "978-951-38-7475-9",
    series = "VTT Science",
    publisher = "VTT Technical Research Centre of Finland",
    number = "14",
    address = "Finland",
    school = "Aalto University",

    }

    Rissanen, A 2012, 'Microsystems for Biological Cell Characterization: Dissertation', Doctor Degree, Aalto University, Espoo.

    Microsystems for Biological Cell Characterization : Dissertation. / Rissanen, Anna.

    Espoo : VTT Technical Research Centre of Finland, 2012. 136 p.

    Research output: ThesisDissertationMonograph

    TY - THES

    T1 - Microsystems for Biological Cell Characterization

    T2 - Dissertation

    AU - Rissanen, Anna

    N1 - Project code: 78365

    PY - 2012

    Y1 - 2012

    N2 - This thesis describes three techniques for the characterization of living cells using micro-electro-mechanical systems (MEMS) based devices. The study of cellular function and structure is essential for bioprocess control, disease diagnosis, patient treatment and drug discovery. Microsystem technology enables characterization of very small samples, minimal use of expensive reagents, testing of multiple samples in parallel, and point-of-care testing, all of which increase throughput and reduce the analysis cost. The three characterization techniques presented in this thesis could be integrated into a microfluidic cellular total analysis system to obtain complementary information of cellular function. The first part of the thesis presents the characterization of bovine adrenal cortex capillary endothelial cells by impedance spectroscopy in a microsystem which was realized using microfabrication techniques. The microsystem consists of a small-volume cell culture area defined on PDMS walls on a glass substrate with gold electrodes coated with a self-assembled monolayer to enable cell attachment. As the main result, it was possible to monitor the capillary formation of BACC endothelial cells in a microsystem using impedance spectroscopy. The second part describes calorimetric characterization of Saccharomyces cerevisiae yeast cells using a MEMS-based nanocalorimetric microsensor. The cells are introduced to the sensor membrane in small droplets (~1 µl), and the sensor thermopile voltage output is compared to the output of the reference water droplet to extract the effect of sample evaporation. The third part describes the design, process integration and fabrication of an electrically tunable Fabry-Perot interferometer (FPI) monolithically integrated on a photodiode for visible spectrum measurements. The options for the process integration of separate FPI optical filters are presented. The application of miniature spectrometers based on MEMS FPI technology in biological cell characterization is discussed.

    AB - This thesis describes three techniques for the characterization of living cells using micro-electro-mechanical systems (MEMS) based devices. The study of cellular function and structure is essential for bioprocess control, disease diagnosis, patient treatment and drug discovery. Microsystem technology enables characterization of very small samples, minimal use of expensive reagents, testing of multiple samples in parallel, and point-of-care testing, all of which increase throughput and reduce the analysis cost. The three characterization techniques presented in this thesis could be integrated into a microfluidic cellular total analysis system to obtain complementary information of cellular function. The first part of the thesis presents the characterization of bovine adrenal cortex capillary endothelial cells by impedance spectroscopy in a microsystem which was realized using microfabrication techniques. The microsystem consists of a small-volume cell culture area defined on PDMS walls on a glass substrate with gold electrodes coated with a self-assembled monolayer to enable cell attachment. As the main result, it was possible to monitor the capillary formation of BACC endothelial cells in a microsystem using impedance spectroscopy. The second part describes calorimetric characterization of Saccharomyces cerevisiae yeast cells using a MEMS-based nanocalorimetric microsensor. The cells are introduced to the sensor membrane in small droplets (~1 µl), and the sensor thermopile voltage output is compared to the output of the reference water droplet to extract the effect of sample evaporation. The third part describes the design, process integration and fabrication of an electrically tunable Fabry-Perot interferometer (FPI) monolithically integrated on a photodiode for visible spectrum measurements. The options for the process integration of separate FPI optical filters are presented. The application of miniature spectrometers based on MEMS FPI technology in biological cell characterization is discussed.

    KW - BioMEMS

    KW - impedance spectroscopy

    KW - MEMS nanocalorimeter

    KW - Fabry-Perot interferometer

    KW - microspectrometers

    KW - Saccharomyces cerevisiae

    KW - yeast

    KW - cell measurement

    M3 - Dissertation

    SN - 978-951-38-7475-9

    T3 - VTT Science

    PB - VTT Technical Research Centre of Finland

    CY - Espoo

    ER -

    Rissanen A. Microsystems for Biological Cell Characterization: Dissertation. Espoo: VTT Technical Research Centre of Finland, 2012. 136 p.