Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes

Adelina Orellana, David Mottershead, Inge van der Linden, Kari Keinänen, Christian Oker-Blom (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The envelope glycoproteins E1 and E2 of rubella virus (RV) were engineered to display the FLAG epitope tag and a polyhistidine tag, at their amino and carboxy termini, respectively. These modified envelope proteins were produced in Sf9 insect cells utilizing baculovirus expression vectors, the E1 and E2 vectors giving rise to protein products of about 58 and 42 kDa, respectively. The recombinant proteins were purified by immobilized metal-ion affinity chromatography and reconstituted into liposomes via their hydrophobic transmembrane anchors. The liposomes were prepared by detergent dialysis in the presence of europium-DTPA chelate, enabling the subsequent measurement of the binding of the resultant proteoliposomes to the antibodies by time resolved fluorescence. RV mimicking proteoliposomes were recognized by antibodies specific for the E1 and E2 proteins, as well as the FLAG epitope tag. This type of virosome may prove useful for studies on the basic biological events of an RV infection or as diagnostic reagents.
Original languageEnglish
Pages (from-to)209-219
Number of pages11
JournalJournal of Biotechnology
Volume75
Issue number2-3
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

Rubella virus
Glycoproteins
Liposomes
Viruses
Virion
Epitopes
Proteins
Antibodies
Virosomes
Europium
Affinity chromatography
Sf9 Cells
Ion chromatography
Recombinant proteins
Pentetic Acid
Dialysis
Baculoviridae
Detergents
Virus Diseases
Anchors

Cite this

Orellana, A., Mottershead, D., van der Linden, I., Keinänen, K., & Oker-Blom, C. (1999). Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes. Journal of Biotechnology, 75(2-3), 209-219. https://doi.org/10.1016/S0168-1656(99)00162-5
Orellana, Adelina ; Mottershead, David ; van der Linden, Inge ; Keinänen, Kari ; Oker-Blom, Christian. / Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes. In: Journal of Biotechnology. 1999 ; Vol. 75, No. 2-3. pp. 209-219.
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abstract = "The envelope glycoproteins E1 and E2 of rubella virus (RV) were engineered to display the FLAG epitope tag and a polyhistidine tag, at their amino and carboxy termini, respectively. These modified envelope proteins were produced in Sf9 insect cells utilizing baculovirus expression vectors, the E1 and E2 vectors giving rise to protein products of about 58 and 42 kDa, respectively. The recombinant proteins were purified by immobilized metal-ion affinity chromatography and reconstituted into liposomes via their hydrophobic transmembrane anchors. The liposomes were prepared by detergent dialysis in the presence of europium-DTPA chelate, enabling the subsequent measurement of the binding of the resultant proteoliposomes to the antibodies by time resolved fluorescence. RV mimicking proteoliposomes were recognized by antibodies specific for the E1 and E2 proteins, as well as the FLAG epitope tag. This type of virosome may prove useful for studies on the basic biological events of an RV infection or as diagnostic reagents.",
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Orellana, A, Mottershead, D, van der Linden, I, Keinänen, K & Oker-Blom, C 1999, 'Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes', Journal of Biotechnology, vol. 75, no. 2-3, pp. 209-219. https://doi.org/10.1016/S0168-1656(99)00162-5

Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes. / Orellana, Adelina; Mottershead, David; van der Linden, Inge; Keinänen, Kari; Oker-Blom, Christian (Corresponding Author).

In: Journal of Biotechnology, Vol. 75, No. 2-3, 1999, p. 209-219.

Research output: Contribution to journalArticleScientificpeer-review

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