Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis

Yunfeng Nie (Corresponding Author), Uusitalo Sanna, Teemu Sipola, Annukka Kokkonen, Inka Päkkilä, Juha Sumen, Katariina Rahkamaa-Tolonen, Volodymyr Tkachenko, Veronica Vespini, Sara Coppola, Pietro Ferraro, Simonetta Grilli, Heidi Ottevaere

Research output: Contribution to journalArticleScientificpeer-review

Abstract

To meet the growing demand for early fatal disease screening among large populations, current fluorescence detection instruments aiming at point-of-care diagnosis have the tendency to be low cost and high sensitivity, with a high potential for the analysis of low-volume, multiplex analytes with easy operation. In this work, we present the development of a miniaturized, high numerical aperture confocal fluorescence scanner for sub-micro-liter fluid diagnosis. It is enhanced with high-rate analyte accumulation using a pyroelectro-hydrodynamic dispensing system for generating tiny, stable sample droplets. The simplified confocal fluorescence scanner (numerical aperture 0.79, working distance 7.3 mm) uses merely off-the-shelf mass-production optical components. Experimental results show that it can achieve a high-sensitive, cost-efficient detection for sub-micro-liter, low-abundant (0.04 µL, 0.67 attomoles) fluid diagnosis, promising for point-of-care diagnosis.

Original languageEnglish
Pages (from-to)6138-6150
Number of pages13
JournalBiomedical Optics Express
Volume14
Issue number12
DOIs
Publication statusPublished - 2023
MoE publication typeA1 Journal article-refereed

Funding

Funding. Academy of Finland (PREIN 346545); Fonds Wetenschappelijk Onderzoek (1252722N); Vrije Universiteit Brussel (Hercules, Methusalem, OZR); European Research Executive Agency (829104).

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