The need to control physical and chemical phenomena requires on-line instruments or modifications of existing commercial instruments to on-line purposes. Capillary electrophoresis (CE) has demonstrated its potential as a significant technique for a variety of analyses due to especially its high speed and ease of automation. In this study, we have developed, built and tested an on-line CE instrument, which is suitable for on-line control of indutrial processes. The instrument is constructed from two identical solvent modules for electrolyte solutions and sample made of PMMA polymer. They are on-line coupled with the ends of an installation module designed for a capillary and an UV or a LIF detector. A peristaltic pump is used to feed the solutions. Samples are injected with the aid of a loop and electrical actuated valve. A thermostated box for the whole module system and the solvent reservoirs, an UV detector, computer controller and data processing units are modified for the instrument. The peristaltic pump, a sampling valve and the on-line modules are connected with Teflon tubes to a 6-way low pressure selection valve allowing selection of solvents for conditioning, rinsing and separation stages. The miniaturized on-line CE system has been used in monitoring water-soluble anions in pulp and paper processes at mills.
|Title of host publication||Finnish chemical congress and exhibition|
|Subtitle of host publication||Miniaturised total analysis systems|
|Place of Publication||Helsinki|
|Publication status||Published - 2002|
|MoE publication type||B3 Non-refereed article in conference proceedings|
|Event||Finnish chemical congress and exhibition - Helsinki, Finland|
Duration: 12 Nov 2002 → 14 Nov 2002
|Conference||Finnish chemical congress and exhibition|
|Period||12/11/02 → 14/11/02|
- on-line capillary electrophoresis
- industrial processes
- pulp waters
Siren, H., Rovio, S., Työppönen, T., Vastamäki, P., Hokkanen, A., & Savolahti, P. (2002). Miniaturized on-line capillary electrophoresis instrument. In Finnish chemical congress and exhibition: Miniaturised total analysis systems .