Miniaturized on-line capillary electrophoresis instrument

Heli Siren, Stella Rovio, Teemu Työppönen, Pertti Vastamäki, Ari Hokkanen, Pekka Savolahti

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

Abstract

The need to control physical and chemical phenomena requires on-line instruments or modifications of existing commercial instruments to on-line purposes. Capillary electrophoresis (CE) has demonstrated its potential as a significant technique for a variety of analyses due to especially its high speed and ease of automation. In this study, we have developed, built and tested an on-line CE instrument, which is suitable for on-line control of indutrial processes. The instrument is constructed from two identical solvent modules for electrolyte solutions and sample made of PMMA polymer. They are on-line coupled with the ends of an installation module designed for a capillary and an UV or a LIF detector. A peristaltic pump is used to feed the solutions. Samples are injected with the aid of a loop and electrical actuated valve. A thermostated box for the whole module system and the solvent reservoirs, an UV detector, computer controller and data processing units are modified for the instrument. The peristaltic pump, a sampling valve and the on-line modules are connected with Teflon tubes to a 6-way low pressure selection valve allowing selection of solvents for conditioning, rinsing and separation stages. The miniaturized on-line CE system has been used in monitoring water-soluble anions in pulp and paper processes at mills.
Original languageEnglish
Title of host publicationFinnish chemical congress and exhibition
Subtitle of host publicationMiniaturised total analysis systems
Place of PublicationHelsinki
Publication statusPublished - 2002
MoE publication typeB3 Non-refereed article in conference proceedings
EventFinnish chemical congress and exhibition - Helsinki, Finland
Duration: 12 Nov 200214 Nov 2002

Conference

ConferenceFinnish chemical congress and exhibition
CountryFinland
CityHelsinki
Period12/11/0214/11/02

Fingerprint

Capillary electrophoresis
Pumps
Ultraviolet detectors
Polytetrafluoroethylene
Polymethyl Methacrylate
Electrolytes
Pulp
Anions
Polymers
Automation
Sampling
Detectors
Controllers
Water
Monitoring

Keywords

  • on-line capillary electrophoresis
  • industrial processes
  • pulp waters
  • anions

Cite this

Siren, H., Rovio, S., Työppönen, T., Vastamäki, P., Hokkanen, A., & Savolahti, P. (2002). Miniaturized on-line capillary electrophoresis instrument. In Finnish chemical congress and exhibition: Miniaturised total analysis systems [29] Helsinki.
Siren, Heli ; Rovio, Stella ; Työppönen, Teemu ; Vastamäki, Pertti ; Hokkanen, Ari ; Savolahti, Pekka. / Miniaturized on-line capillary electrophoresis instrument. Finnish chemical congress and exhibition: Miniaturised total analysis systems. Helsinki, 2002.
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Siren, H, Rovio, S, Työppönen, T, Vastamäki, P, Hokkanen, A & Savolahti, P 2002, Miniaturized on-line capillary electrophoresis instrument. in Finnish chemical congress and exhibition: Miniaturised total analysis systems., 29, Helsinki, Finnish chemical congress and exhibition, Helsinki, Finland, 12/11/02.

Miniaturized on-line capillary electrophoresis instrument. / Siren, Heli; Rovio, Stella; Työppönen, Teemu; Vastamäki, Pertti; Hokkanen, Ari; Savolahti, Pekka.

Finnish chemical congress and exhibition: Miniaturised total analysis systems. Helsinki, 2002. 29.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

TY - GEN

T1 - Miniaturized on-line capillary electrophoresis instrument

AU - Siren, Heli

AU - Rovio, Stella

AU - Työppönen, Teemu

AU - Vastamäki, Pertti

AU - Hokkanen, Ari

AU - Savolahti, Pekka

N1 - CA2: PRO3 CA: PRO

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N2 - The need to control physical and chemical phenomena requires on-line instruments or modifications of existing commercial instruments to on-line purposes. Capillary electrophoresis (CE) has demonstrated its potential as a significant technique for a variety of analyses due to especially its high speed and ease of automation. In this study, we have developed, built and tested an on-line CE instrument, which is suitable for on-line control of indutrial processes. The instrument is constructed from two identical solvent modules for electrolyte solutions and sample made of PMMA polymer. They are on-line coupled with the ends of an installation module designed for a capillary and an UV or a LIF detector. A peristaltic pump is used to feed the solutions. Samples are injected with the aid of a loop and electrical actuated valve. A thermostated box for the whole module system and the solvent reservoirs, an UV detector, computer controller and data processing units are modified for the instrument. The peristaltic pump, a sampling valve and the on-line modules are connected with Teflon tubes to a 6-way low pressure selection valve allowing selection of solvents for conditioning, rinsing and separation stages. The miniaturized on-line CE system has been used in monitoring water-soluble anions in pulp and paper processes at mills.

AB - The need to control physical and chemical phenomena requires on-line instruments or modifications of existing commercial instruments to on-line purposes. Capillary electrophoresis (CE) has demonstrated its potential as a significant technique for a variety of analyses due to especially its high speed and ease of automation. In this study, we have developed, built and tested an on-line CE instrument, which is suitable for on-line control of indutrial processes. The instrument is constructed from two identical solvent modules for electrolyte solutions and sample made of PMMA polymer. They are on-line coupled with the ends of an installation module designed for a capillary and an UV or a LIF detector. A peristaltic pump is used to feed the solutions. Samples are injected with the aid of a loop and electrical actuated valve. A thermostated box for the whole module system and the solvent reservoirs, an UV detector, computer controller and data processing units are modified for the instrument. The peristaltic pump, a sampling valve and the on-line modules are connected with Teflon tubes to a 6-way low pressure selection valve allowing selection of solvents for conditioning, rinsing and separation stages. The miniaturized on-line CE system has been used in monitoring water-soluble anions in pulp and paper processes at mills.

KW - on-line capillary electrophoresis

KW - industrial processes

KW - pulp waters

KW - anions

M3 - Conference article in proceedings

BT - Finnish chemical congress and exhibition

CY - Helsinki

ER -

Siren H, Rovio S, Työppönen T, Vastamäki P, Hokkanen A, Savolahti P. Miniaturized on-line capillary electrophoresis instrument. In Finnish chemical congress and exhibition: Miniaturised total analysis systems. Helsinki. 2002. 29