Miniaturized on-line capillary electrophoresis instrument

Heli Siren, Stella Rovio, Teemu Työppönen, Pertti Vastamäki, Ari Hokkanen, Pekka Savolahti

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

    Abstract

    The need to control physical and chemical phenomena requires on-line instruments or modifications of existing commercial instruments to on-line purposes. Capillary electrophoresis (CE) has demonstrated its potential as a significant technique for a variety of analyses due to especially its high speed and ease of automation. In this study, we have developed, built and tested an on-line CE instrument, which is suitable for on-line control of indutrial processes. The instrument is constructed from two identical solvent modules for electrolyte solutions and sample made of PMMA polymer. They are on-line coupled with the ends of an installation module designed for a capillary and an UV or a LIF detector. A peristaltic pump is used to feed the solutions. Samples are injected with the aid of a loop and electrical actuated valve. A thermostated box for the whole module system and the solvent reservoirs, an UV detector, computer controller and data processing units are modified for the instrument. The peristaltic pump, a sampling valve and the on-line modules are connected with Teflon tubes to a 6-way low pressure selection valve allowing selection of solvents for conditioning, rinsing and separation stages. The miniaturized on-line CE system has been used in monitoring water-soluble anions in pulp and paper processes at mills.
    Original languageEnglish
    Title of host publicationFinnish chemical congress and exhibition
    Subtitle of host publicationMiniaturised total analysis systems
    Place of PublicationHelsinki
    Publication statusPublished - 2002
    MoE publication typeB3 Non-refereed article in conference proceedings
    EventFinnish chemical congress and exhibition - Helsinki, Finland
    Duration: 12 Nov 200214 Nov 2002

    Conference

    ConferenceFinnish chemical congress and exhibition
    CountryFinland
    CityHelsinki
    Period12/11/0214/11/02

    Keywords

    • on-line capillary electrophoresis
    • industrial processes
    • pulp waters
    • anions

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