Miscibility in binary monolayers of phospholipids and linker lipid

Erica Györvary (Corresponding Author), Willem M. Albers, Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

We studied the miscibility in binary lipid matrixes made by the Langmuir−Blodgett (LB) technique. The components in the lipid matrix were N-(ε-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used:  1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure−area isotherms. On the contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.
Original languageEnglish
Pages (from-to)2516-2524
Number of pages9
JournalLangmuir
Volume15
Issue number7
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Phospholipids
Lipids
lipids
Electromagnetic compatibility
Monolayers
solubility
Solubility
matrices
Brewster angle
Microscopic examination
microscopy
1,2-Dipalmitoylphosphatidylcholine
monomolecular films
Surface potential
Phosphatidylcholines
Superconducting transition temperature
Fourier transform infrared spectroscopy
Isotherms
Atomic force microscopy
isotherms

Cite this

Györvary, E., Albers, W. M., & Peltonen, J. (1999). Miscibility in binary monolayers of phospholipids and linker lipid. Langmuir, 15(7), 2516-2524. https://doi.org/10.1021/la981253x
Györvary, Erica ; Albers, Willem M. ; Peltonen, Jouko. / Miscibility in binary monolayers of phospholipids and linker lipid. In: Langmuir. 1999 ; Vol. 15, No. 7. pp. 2516-2524.
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abstract = "We studied the miscibility in binary lipid matrixes made by the Langmuir−Blodgett (LB) technique. The components in the lipid matrix were N-(ε-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used:  1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure−area isotherms. On the contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.",
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Györvary, E, Albers, WM & Peltonen, J 1999, 'Miscibility in binary monolayers of phospholipids and linker lipid', Langmuir, vol. 15, no. 7, pp. 2516-2524. https://doi.org/10.1021/la981253x

Miscibility in binary monolayers of phospholipids and linker lipid. / Györvary, Erica (Corresponding Author); Albers, Willem M.; Peltonen, Jouko.

In: Langmuir, Vol. 15, No. 7, 1999, p. 2516-2524.

Research output: Contribution to journalArticleScientificpeer-review

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N2 - We studied the miscibility in binary lipid matrixes made by the Langmuir−Blodgett (LB) technique. The components in the lipid matrix were N-(ε-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used:  1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure−area isotherms. On the contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.

AB - We studied the miscibility in binary lipid matrixes made by the Langmuir−Blodgett (LB) technique. The components in the lipid matrix were N-(ε-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used:  1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure−area isotherms. On the contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.

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