Mitochondrial metabolomics in Saccharomyces cerevisiae

Eija Rintala, Matej Oresic, Helena Simolin, Ismo Mattila, Hannu Maaheimo, Laura Ruohonen, Merja Penttilä

Research output: Contribution to conferenceConference articleScientific

Abstract

Mitochondria play a key role in the energy metabolism and redox control of the cell. They also have important functions in apoptosis and the metabolism of amino acids, lipids and iron. Recently, the knowledge about mitochondria have been widened by proteomic and transcriptional studies, but a lot still remains unknown. We are therefore aiming to study the mitochondrial metabolism of Saccharomyces cerevisiae as a whole and compare it to overall cellular metabolism. We are going to do metabolic profiling of isolated mitochondria by using LC/MS, GC/MS and NMR methods. New computational and statistical methods are developed and applied to tract subtle changes in spectral data, which leads to identification of compounds most descriptive of various physiological responses. We have already made preliminary studies on glucose grown yeast cells and have been able to show differences between the mitochondrial and whole cell metabolites. Based on 2627 peaks detected using LC/MS we could separate the metabolites with principle component analysis; with 140 peaks we could differentiate the compartments with p>0.05. Ultimately, we are aiming to relate these studies to the physiological status of the cell by comparing the data from metabolite level with transcriptional and proteomic data. We believe that this may reveal us a more complete picture of the mitochondria and at the same time the whole metabolism occurring in the yeast cell.
Original languageEnglish
Publication statusPublished - 2004
EventMetabolic Engineering V: Genome to Product - Lake Tahoe, United States
Duration: 19 Sep 200423 Sep 2004

Conference

ConferenceMetabolic Engineering V
CountryUnited States
CityLake Tahoe
Period19/09/0423/09/04

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Rintala, E., Oresic, M., Simolin, H., Mattila, I., Maaheimo, H., Ruohonen, L., & Penttilä, M. (2004). Mitochondrial metabolomics in Saccharomyces cerevisiae. Paper presented at Metabolic Engineering V, Lake Tahoe, United States.