Mixotrophic continuous flow cultivation of Chlorella protothecoides for lipids

Yanming Wang, Heiko Rischer, N.T. Eriksen, Marilyn Wiebe (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

The oleaginous alga Chlorella protothecoides accumulates lipid in its biomass when grown in nitrogen-restricted conditions. To assess the relationship between nitrogen provision and lipid accumulation and to determine the contribution of photosynthesis in mixotrophic growth, C. protothecoides was grown in mixo- and heterotrophic nitrogen-limited continuous flow cultures. Lipid content increased with decreasing C/N, while biomass yield on glucose was not affected. Continuous production of high lipid levels (57% of biomass) was possible at high C/N (87–94). However, the lipid production rate (2.48 g L−1 d−1) was higher at D = 0.84 d−1 with C/N 37 than at D = 0.44 d−1 and C/N 87 even though the lipid content of the biomass was lower (38%). Photosynthesis contributed to biomass and lipid production in mixotrophic conditions, resulting in 13–38% reduction in CO2 production compared with heterotrophic cultures, demonstrating that photo- and heterotrophic growth occurred simultaneously in the same population.
Original languageEnglish
Pages (from-to)608-614
Number of pages6
JournalBioresource Technology
Volume144
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Lipids
lipid
Biomass
biomass
Nitrogen
Photosynthesis
nitrogen
photosynthesis
Algae
Glucose
glucose
alga

Keywords

  • C/N ratio
  • continuous flow culture
  • lipid
  • microalgae
  • mixotrophic growth

Cite this

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title = "Mixotrophic continuous flow cultivation of Chlorella protothecoides for lipids",
abstract = "The oleaginous alga Chlorella protothecoides accumulates lipid in its biomass when grown in nitrogen-restricted conditions. To assess the relationship between nitrogen provision and lipid accumulation and to determine the contribution of photosynthesis in mixotrophic growth, C. protothecoides was grown in mixo- and heterotrophic nitrogen-limited continuous flow cultures. Lipid content increased with decreasing C/N, while biomass yield on glucose was not affected. Continuous production of high lipid levels (57{\%} of biomass) was possible at high C/N (87–94). However, the lipid production rate (2.48 g L−1 d−1) was higher at D = 0.84 d−1 with C/N 37 than at D = 0.44 d−1 and C/N 87 even though the lipid content of the biomass was lower (38{\%}). Photosynthesis contributed to biomass and lipid production in mixotrophic conditions, resulting in 13–38{\%} reduction in CO2 production compared with heterotrophic cultures, demonstrating that photo- and heterotrophic growth occurred simultaneously in the same population.",
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author = "Yanming Wang and Heiko Rischer and N.T. Eriksen and Marilyn Wiebe",
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Mixotrophic continuous flow cultivation of Chlorella protothecoides for lipids. / Wang, Yanming; Rischer, Heiko; Eriksen, N.T.; Wiebe, Marilyn (Corresponding Author).

In: Bioresource Technology, Vol. 144, 2013, p. 608-614.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Mixotrophic continuous flow cultivation of Chlorella protothecoides for lipids

AU - Wang, Yanming

AU - Rischer, Heiko

AU - Eriksen, N.T.

AU - Wiebe, Marilyn

PY - 2013

Y1 - 2013

N2 - The oleaginous alga Chlorella protothecoides accumulates lipid in its biomass when grown in nitrogen-restricted conditions. To assess the relationship between nitrogen provision and lipid accumulation and to determine the contribution of photosynthesis in mixotrophic growth, C. protothecoides was grown in mixo- and heterotrophic nitrogen-limited continuous flow cultures. Lipid content increased with decreasing C/N, while biomass yield on glucose was not affected. Continuous production of high lipid levels (57% of biomass) was possible at high C/N (87–94). However, the lipid production rate (2.48 g L−1 d−1) was higher at D = 0.84 d−1 with C/N 37 than at D = 0.44 d−1 and C/N 87 even though the lipid content of the biomass was lower (38%). Photosynthesis contributed to biomass and lipid production in mixotrophic conditions, resulting in 13–38% reduction in CO2 production compared with heterotrophic cultures, demonstrating that photo- and heterotrophic growth occurred simultaneously in the same population.

AB - The oleaginous alga Chlorella protothecoides accumulates lipid in its biomass when grown in nitrogen-restricted conditions. To assess the relationship between nitrogen provision and lipid accumulation and to determine the contribution of photosynthesis in mixotrophic growth, C. protothecoides was grown in mixo- and heterotrophic nitrogen-limited continuous flow cultures. Lipid content increased with decreasing C/N, while biomass yield on glucose was not affected. Continuous production of high lipid levels (57% of biomass) was possible at high C/N (87–94). However, the lipid production rate (2.48 g L−1 d−1) was higher at D = 0.84 d−1 with C/N 37 than at D = 0.44 d−1 and C/N 87 even though the lipid content of the biomass was lower (38%). Photosynthesis contributed to biomass and lipid production in mixotrophic conditions, resulting in 13–38% reduction in CO2 production compared with heterotrophic cultures, demonstrating that photo- and heterotrophic growth occurred simultaneously in the same population.

KW - C/N ratio

KW - continuous flow culture

KW - lipid

KW - microalgae

KW - mixotrophic growth

U2 - 10.1016/j.biortech.2013.07.027

DO - 10.1016/j.biortech.2013.07.027

M3 - Article

VL - 144

SP - 608

EP - 614

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -